Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2285081..2285735 Replicon chromosome
Accession NZ_CP087564
Organism Escherichia coli strain CM2010-0578/1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LOF66_RS11585 Protein ID WP_000244777.1
Coordinates 2285081..2285488 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LOF66_RS11590 Protein ID WP_000354046.1
Coordinates 2285469..2285735 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOF66_RS11565 (2281038) 2281038..2282771 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
LOF66_RS11570 (2282777) 2282777..2283487 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LOF66_RS11575 (2283512) 2283512..2284408 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LOF66_RS11580 (2284520) 2284520..2285041 + 522 WP_001055874.1 flavodoxin FldB -
LOF66_RS11585 (2285081) 2285081..2285488 - 408 WP_000244777.1 protein YgfX Toxin
LOF66_RS11590 (2285469) 2285469..2285735 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LOF66_RS11595 (2285978) 2285978..2286958 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LOF66_RS11600 (2287154) 2287154..2287813 - 660 WP_000250274.1 hemolysin III family protein -
LOF66_RS11605 (2287977) 2287977..2288288 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LOF66_RS11610 (2288333) 2288333..2289766 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LOF66_RS11615 (2289823) 2289823..2290566 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T225488 WP_000244777.1 NZ_CP087564:c2285488-2285081 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T225488 NZ_CP121153:c2171919-2171812 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT225488 WP_000354046.1 NZ_CP087564:c2285735-2285469 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT225488 NZ_CP121153:2171966-2172032 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References