Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 2027459..2028291 | Replicon | chromosome |
| Accession | NZ_CP087564 | ||
| Organism | Escherichia coli strain CM2010-0578/1 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | F4TJC5 |
| Locus tag | LOF66_RS10345 | Protein ID | WP_001094452.1 |
| Coordinates | 2027917..2028291 (+) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | F4TJC4 |
| Locus tag | LOF66_RS10340 | Protein ID | WP_001285579.1 |
| Coordinates | 2027459..2027827 (+) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOF66_RS10320 (2025208) | 2025208..2026026 | + | 819 | WP_001564116.1 | DUF932 domain-containing protein | - |
| LOF66_RS10325 (2026118) | 2026118..2026603 | + | 486 | WP_000206669.1 | antirestriction protein | - |
| LOF66_RS10330 (2026619) | 2026619..2027095 | + | 477 | WP_001186706.1 | RadC family protein | - |
| LOF66_RS10335 (2027158) | 2027158..2027379 | + | 222 | WP_000692345.1 | DUF987 domain-containing protein | - |
| LOF66_RS10340 (2027459) | 2027459..2027827 | + | 369 | WP_001285579.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| LOF66_RS10345 (2027917) | 2027917..2028291 | + | 375 | WP_001094452.1 | TA system toxin CbtA family protein | Toxin |
| LOF66_RS10350 (2028288) | 2028288..2028779 | + | 492 | WP_000976868.1 | DUF5983 family protein | - |
| LOF66_RS10355 (2028791) | 2028791..2028988 | + | 198 | WP_000772035.1 | DUF957 domain-containing protein | - |
| LOF66_RS10360 (2029085) | 2029085..2029930 | + | 846 | WP_021539310.1 | DUF4942 domain-containing protein | - |
| LOF66_RS10370 (2030209) | 2030209..2031372 | + | 1164 | Protein_2038 | IS3-like element IS3 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14014.86 Da Isoelectric Point: 7.2920
>T225486 WP_001094452.1 NZ_CP087564:2027917-2028291 [Escherichia coli]
MNTLPDTHVREASRCSSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSTDILRARRATGLMTRDNYRTVNNITLGKHPEAK
MNTLPDTHVREASRCSSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSTDILRARRATGLMTRDNYRTVNNITLGKHPEAK
Download Length: 375 bp
>T225486 NZ_CP121153:c2170848-2170741 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA
Antitoxin
Download Length: 123 a.a. Molecular weight: 13580.39 Da Isoelectric Point: 7.3223
>AT225486 WP_001285579.1 NZ_CP087564:2027459-2027827 [Escherichia coli]
VSDTLPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYLAVYPTSETKK
VSDTLPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYLAVYPTSETKK
Download Length: 369 bp
>AT225486 NZ_CP121153:2170896-2170962 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7Z7YIM6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7Z8DWX2 |