Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 745924..746450 Replicon chromosome
Accession NZ_CP087564
Organism Escherichia coli strain CM2010-0578/1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LOF66_RS03820 Protein ID WP_000323025.1
Coordinates 745924..746211 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LOF66_RS03825 Protein ID WP_000534858.1
Coordinates 746211..746450 (-) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOF66_RS03760 (741073) 741073..741570 - 498 WP_001071769.1 DUF2514 domain-containing protein -
LOF66_RS03765 (741567) 741567..742100 - 534 WP_001092971.1 lysozyme -
LOF66_RS03770 (742097) 742097..742408 - 312 WP_000189916.1 YdfR family protein -
LOF66_RS03775 (742413) 742413..742628 - 216 WP_000839590.1 phage lysis protein EssD -
LOF66_RS03780 (742848) 742848..743018 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
LOF66_RS03785 (743382) 743382..743597 - 216 WP_000066484.1 cold shock-like protein CspB -
LOF66_RS03790 (743898) 743898..744110 + 213 WP_000087756.1 cold shock-like protein CspF -
LOF66_RS03795 (744165) 744165..744254 + 90 WP_120795389.1 hypothetical protein -
LOF66_RS03800 (744532) 744532..744855 - 324 Protein_750 antitermination protein -
LOF66_RS03805 (744884) 744884..745162 - 279 WP_012304870.1 hypothetical protein -
LOF66_RS03810 (745229) 745229..745480 - 252 WP_000980994.1 protein Rem -
LOF66_RS03815 (745697) 745697..745852 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LOF66_RS03820 (745924) 745924..746211 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LOF66_RS03825 (746211) 746211..746450 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LOF66_RS03830 (746475) 746475..746780 + 306 WP_001326990.1 protein YdfV -
LOF66_RS03835 (746983) 746983..747315 + 333 WP_001301033.1 FlxA-like family protein -
LOF66_RS03840 (747752) 747752..747901 - 150 WP_011443592.1 protein YdfW -
LOF66_RS03845 (748022) 748022..749044 - 1023 Protein_759 ISNCY family transposase -
LOF66_RS03850 (749834) 749834..750121 - 288 Protein_760 hypothetical protein -
LOF66_RS03855 (750599) 750599..751088 - 490 Protein_761 class I SAM-dependent methyltransferase -
LOF66_RS03860 (751085) 751085..751336 - 252 Protein_762 DUF977 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 736037..759916 23879


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T225475 WP_000323025.1 NZ_CP087564:c746211-745924 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T225475 NZ_CP121152:115811-116098 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT225475 WP_000534858.1 NZ_CP087564:c746450-746211 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT225475 NZ_CP121152:115563-115814 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References