Detailed information of TA system TA225473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 606132..606770
• Replicon: chromosome
• Accession: NZ_CP087564
• Organism: Escherichia coli strain CM2010-0578/1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LOF66_RS03125
• Protein ID: WP_000813794.1
• Coordinates: 606132..606308 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOF66_RS03130
• Protein ID: WP_001270286.1
• Coordinates: 606354..606770 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOF66_RS03105 (601751)	601751..602926	-	1176	WP_001236265.1	BenE family transporter YdcO	-
LOF66_RS03110 (603018)	603018..603554	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LOF66_RS03115 (603627)	603627..605588	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LOF66_RS03120 (605680)	605680..605910	-	231	WP_000494244.1	YncJ family protein	-
LOF66_RS03125 (606132)	606132..606308	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LOF66_RS03130 (606354)	606354..606770	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LOF66_RS03135 (606849)	606849..608255	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LOF66_RS03140 (608500)	608500..609645	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LOF66_RS03145 (609663)	609663..610676	+	1014	WP_062897062.1	ABC transporter ATP-binding protein	-
LOF66_RS03150 (610677)	610677..611618	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T225473 WP_000813794.1 NZ_CP087564:606132-606308 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T225473 NZ_CP121152:61171-61452 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT225473 WP_001270286.1 NZ_CP087564:606354-606770 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT225473 NZ_CP121152:60930-61181 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• PDB: 6HPB