Detailed information of TA system TA225468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 4425842..4426681
• Replicon: chromosome
• Accession: NZ_CP087392
• Organism: Pectobacterium parvum strain FN20211

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6P1S3C2
• Locus tag: LOZ86_RS19950
• Protein ID: WP_039482128.1
• Coordinates: 4425842..4426204 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOZ86_RS19955
• Protein ID: WP_039482130.1
• Coordinates: 4426208..4426681 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ86_RS19930 (LOZ86_19930)	4421210..4421944	-	735	WP_111778762.1	type I-E CRISPR-associated protein Cas5/CasD	-
LOZ86_RS19935 (LOZ86_19935)	4421954..4423021	-	1068	WP_039482121.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
LOZ86_RS19940 (LOZ86_19940)	4423025..4423672	-	648	WP_039482124.1	type I-E CRISPR-associated protein Cse2/CasB	-
LOZ86_RS19945 (LOZ86_19945)	4423665..4425227	-	1563	WP_039482127.1	type I-E CRISPR-associated protein Cse1/CasA	-
LOZ86_RS19950 (LOZ86_19950)	4425842..4426204	+	363	WP_039482128.1	type II toxin-antitoxin system HigB family toxin	Toxin
LOZ86_RS19955 (LOZ86_19955)	4426208..4426681	+	474	WP_039482130.1	helix-turn-helix domain-containing protein	Antitoxin
LOZ86_RS19960 (LOZ86_19960)	4426671..4429388	-	2718	WP_052012030.1	CRISPR-associated helicase/endonuclease Cas3	-
LOZ86_RS19965 (LOZ86_19965)	4429420..4429971	-	552	WP_039482132.1	polymer-forming cytoskeletal protein	-
LOZ86_RS19970 (LOZ86_19970)	4430141..4430329	-	189	WP_039482134.1	hypothetical protein	-
LOZ86_RS19975 (LOZ86_19975)	4430653..4431576	+	924	WP_039482136.1	aminoimidazole riboside kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4417495..4426681	9186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (74-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (108-154)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14134.36 Da
• Isoelectric Point: 9.7305

>T225468 WP_039482128.1 NZ_CP087392:4425842-4426204 [Pectobacterium parvum]
MRVISAKAITDAMKKHAKWKVGLKLWLDVFDKPSLRFESYEQLCHVWRNTSCWNVDRIPFALLENESRKGPLDIYVFDIN
GNSCRIIAWLNPNTGSLYVKDVCSHAEYDKWWKAQTKTKR

Nucleotide

• Length: 363 bp

>T225468 NZ_CP121151:4016136-4016678 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17776.06 Da
• Isoelectric Point: 4.2547

>AT225468 WP_039482130.1 NZ_CP087392:4426208-4426681 [Pectobacterium parvum]
MRNTSLGSNSSVGISGLIENLPLLLQEASEQLNSLSVLLSACMEPIEDDNDLQERMECINQLYLYSSDVNHIPATFAELI
ADRVYEYEKKNRDVPHVSQAEALAFFIEERGLKQSDLKQIATQSVISDIINGKRTMTLQQVKDFSRYFNVPIETFID

Nucleotide

• Length: 474 bp

>AT225468 NZ_CP121151:4015841-4016152 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A6P1S3C2