Detailed information of TA system TA225465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 4285792..4286534
• Replicon: chromosome
• Accession: NZ_CP087392
• Organism: Pectobacterium parvum strain FN20211

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LOZ86_RS19275
• Protein ID: WP_039481845.1
• Coordinates: 4285792..4286271 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A6P1S083
• Locus tag: LOZ86_RS19280
• Protein ID: WP_039481848.1
• Coordinates: 4286262..4286534 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ86_RS19235 (LOZ86_19235)	4281581..4281841	+	261	WP_039513585.1	AlpA family transcriptional regulator	-
LOZ86_RS19240 (LOZ86_19240)	4281838..4282062	+	225	WP_230242638.1	hypothetical protein	-
LOZ86_RS19245 (LOZ86_19245)	4282071..4282382	+	312	Protein_3754	ash family protein	-
LOZ86_RS19250 (LOZ86_19250)	4282418..4282615	+	198	WP_230243177.1	host cell division inhibitor Icd-like protein	-
LOZ86_RS19255 (LOZ86_19255)	4282608..4282802	+	195	WP_005973630.1	hypothetical protein	-
LOZ86_RS19260 (LOZ86_19260)	4282799..4283065	+	267	WP_039513580.1	hypothetical protein	-
LOZ86_RS19265 (LOZ86_19265)	4283062..4283403	+	342	WP_039488121.1	DUF5375 family protein	-
LOZ86_RS19270 (LOZ86_19270)	4283414..4285738	+	2325	WP_230242640.1	phage/plasmid primase, P4 family	-
LOZ86_RS19275 (LOZ86_19275)	4285792..4286271	-	480	WP_039481845.1	GNAT family N-acetyltransferase	Toxin
LOZ86_RS19280 (LOZ86_19280)	4286262..4286534	-	273	WP_039481848.1	DUF1778 domain-containing protein	Antitoxin
LOZ86_RS19285 (LOZ86_19285)	4286825..4287076	+	252	WP_005973616.1	prevent-host-death protein	-
LOZ86_RS19290 (LOZ86_19290)	4287066..4287345	+	280	Protein_3763	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
LOZ86_RS19295 (LOZ86_19295)	4287951..4288481	+	531	Protein_3764	integrase arm-type DNA-binding domain-containing protein	-
LOZ86_RS19300 (LOZ86_19300)	4288928..4289212	-	285	WP_005971242.1	helix-turn-helix transcriptional regulator	-
LOZ86_RS19305 (LOZ86_19305)	4289202..4289531	-	330	WP_039481852.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LOZ86_RS19310 (LOZ86_19310)	4289836..4290252	+	417	Protein_3767	N-acetyltransferase	-
LOZ86_RS19315 (LOZ86_19315)	4290343..4291464	+	1122	WP_039481856.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4275285..4289531	14246

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-90)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17429.04 Da
• Isoelectric Point: 8.9715

>T225465 WP_039481845.1 NZ_CP087392:c4286271-4285792 [Pectobacterium parvum]
VGITAPELLLPQHAVVDFRSSEPSLDDWLKRKALKNQTIGASRTFVVCESGANQVIGFYALATGSVQRQSVSGALRRNMP
DPLPVLVLGRLAVDERYHRRGIGAGLLKDAVLRSQQVAEQVGTKALLVHALSEEAKDFYLHWGFTSSEIQPSTLLLPLW

Nucleotide

• Length: 480 bp

>T225465 NZ_CP121151:3043729-3043836 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10288.77 Da
• Isoelectric Point: 4.7174

>AT225465 WP_039481848.1 NZ_CP087392:c4286534-4286262 [Pectobacterium parvum]
MRTEAKEAPINIRAKSSQRELIDVAAKLLSKSRTDFILEAACREAEEVLLDQRLFLVDESQFDAFITALEKPVATNERLQ
ALLDRKSPWE

Nucleotide

• Length: 273 bp

>AT225465 NZ_CP121151:c3043679-3043616 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A6P1S083