Detailed information of TA system TA225459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1846576..1847129
• Replicon: chromosome
• Accession: NZ_CP087392
• Organism: Pectobacterium parvum strain FN20211

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LOZ86_RS08255
• Protein ID: WP_039513711.1
• Coordinates: 1846815..1847129 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0H3I2T3
• Locus tag: LOZ86_RS08250
• Protein ID: WP_014699357.1
• Coordinates: 1846576..1846812 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ86_RS08240 (LOZ86_08240)	1841600..1844686	+	3087	WP_052237790.1	efflux RND transporter permease subunit	-
LOZ86_RS08245 (LOZ86_08245)	1845311..1846255	+	945	WP_039513709.1	zincin-like metallopeptidase domain-containing protein	-
LOZ86_RS08250 (LOZ86_08250)	1846576..1846812	+	237	WP_014699357.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LOZ86_RS08255 (LOZ86_08255)	1846815..1847129	+	315	WP_039513711.1	CcdB family protein	Toxin
LOZ86_RS08260 (LOZ86_08260)	1847163..1847915	-	753	WP_039513714.1	MobC family replication-relaxation protein	-
LOZ86_RS08265 (LOZ86_08265)	1847924..1849660	-	1737	WP_039513716.1	type IV secretory system conjugative DNA transfer family protein	-
LOZ86_RS08270 (LOZ86_08270)	1850454..1850756	-	303	WP_161547089.1	conjugal transfer protein	-
LOZ86_RS08275 (LOZ86_08275)	1850800..1851816	-	1017	WP_039513719.1	P-type DNA transfer ATPase VirB11	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1827050..1873837	46787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (4-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11489.41 Da
• Isoelectric Point: 7.9859

>T225459 WP_039513711.1 NZ_CP087392:1846815-1847129 [Pectobacterium parvum]
MQSTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPERLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T225459 NZ_CP121151:2184235-2184489 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9004.06 Da
• Isoelectric Point: 6.4610

>AT225459 WP_014699357.1 NZ_CP087392:1846576-1846812 [Pectobacterium parvum]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRYHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT225459 NZ_CP121151:2183987-2184238 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3I2T3