Detailed information of TA system TA225458

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 1563152..1563951
• Replicon: chromosome
• Accession: NZ_CP087392
• Organism: Pectobacterium parvum strain FN20211

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: LOZ86_RS07015
• Protein ID: WP_230242992.1
• Coordinates: 1563152..1563571 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: A0A6P1S5H0
• Locus tag: LOZ86_RS07020
• Protein ID: WP_039482638.1
• Coordinates: 1563568..1563951 (-)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ86_RS06995 (LOZ86_06995)	1558266..1558907	+	642	WP_010281304.1	uridine kinase	-
LOZ86_RS07000 (LOZ86_07000)	1559015..1559596	+	582	WP_039482649.1	dCTP deaminase	-
LOZ86_RS07005 (LOZ86_07005)	1559660..1561493	+	1834	Protein_1341	outer membrane assembly protein AsmA	-
LOZ86_RS07010 (LOZ86_07010)	1561756..1563108	+	1353	WP_230242991.1	anaerobic C4-dicarboxylate transporter DcuC	-
LOZ86_RS07015 (LOZ86_07015)	1563152..1563571	-	420	WP_230242992.1	DUF86 domain-containing protein	Toxin
LOZ86_RS07020 (LOZ86_07020)	1563568..1563951	-	384	WP_039482638.1	nucleotidyltransferase domain-containing protein	Antitoxin
LOZ86_RS07025 (LOZ86_07025)	1564091..1565677	-	1587	WP_039482635.1	TerC family protein	-
LOZ86_RS07030 (LOZ86_07030)	1566411..1567508	+	1098	WP_111779044.1	UDP-N-acetylglucosamine--undecaprenyl-phosphate N-acetylglucosaminephosphotransferase	-
LOZ86_RS07035 (LOZ86_07035)	1567628..1568764	+	1137	WP_230242993.1	polysaccharide export protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (12-130)

Antitoxin

• Polymerase beta, nucleotidyltransferase (4-93)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 16137.56 Da
• Isoelectric Point: 8.5183

>T225458 WP_230242992.1 NZ_CP087392:c1563571-1563152 [Pectobacterium parvum]
MTDILLNKSSTIQRCLRRIREEFDTKEEFRQNFTKQDSVILNIQRACEAAIDIANYLIRIKHLGIPQSSRDSFALLAANQ
IITIDLSDNLQKMVGLRNIAVHDYQALNLDIVIHVISHRLSDFERFIKKVARYSDSQHL

Nucleotide

• Length: 420 bp

>T225458 NZ_CP121151:2183987-2184238 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 14312.49 Da
• Isoelectric Point: 5.2241

>AT225458 WP_039482638.1 NZ_CP087392:c1563951-1563568 [Pectobacterium parvum]
MFDKIVSTLKKQIPDIKIIYLFGSQATGNARADSDVDIAILATRALDPVERWELSNQLAKEVGRDVDLIDLLQASTVLKM
EIVRNGKLLYDAKAAAGEFEMTTLSMYQHLQRERADIIRSFNQDLKA

Nucleotide

• Length: 384 bp

>AT225458 NZ_CP121151:2184235-2184489 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A6P1S5H0