Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4494437..4495176 Replicon chromosome
Accession NZ_CP087390
Organism Enterobacter sp. AN-K1

Toxin (Protein)


Gene name tacT Uniprot ID A0A3L9PBP9
Locus tag LOC22_RS21320 Protein ID WP_003857133.1
Coordinates 4494437..4494922 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag LOC22_RS21325 Protein ID WP_003857131.1
Coordinates 4494910..4495176 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOC22_RS21290 (LOC22_21290) 4489925..4490539 + 615 WP_080472113.1 NUDIX hydrolase -
LOC22_RS21295 (LOC22_21295) 4490723..4491358 - 636 WP_015570517.1 DUF421 domain-containing protein -
LOC22_RS21300 (LOC22_21300) 4491368..4491814 - 447 WP_014069654.1 DUF3290 domain-containing protein -
LOC22_RS21305 (LOC22_21305) 4492131..4492571 - 441 WP_063931324.1 helix-turn-helix domain-containing protein -
LOC22_RS21310 (LOC22_21310) 4492836..4493801 + 966 WP_032648329.1 hypothetical protein -
LOC22_RS21315 (LOC22_21315) 4493817..4494386 + 570 WP_032648331.1 hypothetical protein -
LOC22_RS21320 (LOC22_21320) 4494437..4494922 - 486 WP_003857133.1 GNAT family N-acetyltransferase Toxin
LOC22_RS21325 (LOC22_21325) 4494910..4495176 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
LOC22_RS21330 (LOC22_21330) 4495240..4496169 - 930 WP_230239189.1 LysR family transcriptional regulator -
LOC22_RS21335 (LOC22_21335) 4496299..4497681 + 1383 WP_230239568.1 MFS transporter -
LOC22_RS21340 (LOC22_21340) 4497703..4498698 - 996 WP_017382342.1 DUF2891 domain-containing protein -
LOC22_RS21345 (LOC22_21345) 4498708..4499694 - 987 WP_017382341.1 DUF979 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17540.23 Da        Isoelectric Point: 9.9658

>T225448 WP_003857133.1 NZ_CP087390:c4494922-4494437 [Enterobacter sp. AN-K1]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T225448 NZ_CP121149:c4508998-4508825 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT225448 WP_003857131.1 NZ_CP087390:c4495176-4494910 [Enterobacter sp. AN-K1]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT225448 NZ_CP121149:c4509322-4509026 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3L9PBP9

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References