Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3357488..3358108 Replicon chromosome
Accession NZ_CP087390
Organism Enterobacter sp. AN-K1

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag LOC22_RS15975 Protein ID WP_015571250.1
Coordinates 3357488..3357706 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag LOC22_RS15980 Protein ID WP_006809850.1
Coordinates 3357734..3358108 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOC22_RS15945 (LOC22_15945) 3353500..3353760 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
LOC22_RS15950 (LOC22_15950) 3353763..3353903 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LOC22_RS15955 (LOC22_15955) 3353900..3354610 - 711 WP_230239039.1 GNAT family protein -
LOC22_RS15960 (LOC22_15960) 3354712..3356172 + 1461 WP_045349927.1 PLP-dependent aminotransferase family protein -
LOC22_RS15965 (LOC22_15965) 3356144..3356611 - 468 WP_023296041.1 YlaC family protein -
LOC22_RS15970 (LOC22_15970) 3356728..3357279 - 552 WP_015571251.1 maltose O-acetyltransferase -
LOC22_RS15975 (LOC22_15975) 3357488..3357706 - 219 WP_015571250.1 HHA domain-containing protein Toxin
LOC22_RS15980 (LOC22_15980) 3357734..3358108 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
LOC22_RS15985 (LOC22_15985) 3358619..3361765 - 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit AcrB -
LOC22_RS15990 (LOC22_15990) 3361788..3362981 - 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T225447 WP_015571250.1 NZ_CP087390:c3357706-3357488 [Enterobacter sp. AN-K1]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T225447 NZ_CP121149:c4332709-4332359 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT225447 WP_006809850.1 NZ_CP087390:c3358108-3357734 [Enterobacter sp. AN-K1]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT225447 NZ_CP121149:c4332954-4332703 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References