Detailed information of TA system TA225446

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yfjZ/CbtA-CbeA
• Location: 3207403..3208160
• Replicon: chromosome
• Accession: NZ_CP087390
• Organism: Enterobacter sp. AN-K1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: LOC22_RS15265
• Protein ID: WP_196347929.1
• Coordinates: 3207403..3207786 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LOC22_RS15270
• Protein ID: WP_196347930.1
• Coordinates: 3207822..3208160 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOC22_RS15240 (LOC22_15240)	3202602..3203003	+	402	WP_006809999.1	sigma factor-binding protein Crl	-
LOC22_RS15245 (LOC22_15245)	3203136..3204188	-	1053	WP_017383128.1	phosphoporin PhoE	-
LOC22_RS15250 (LOC22_15250)	3204494..3205597	+	1104	WP_015571369.1	glutamate 5-kinase	-
LOC22_RS15255 (LOC22_15255)	3205609..3206862	+	1254	WP_047728882.1	glutamate-5-semialdehyde dehydrogenase	-
LOC22_RS15265 (LOC22_15265)	3207403..3207786	-	384	WP_196347929.1	TA system toxin CbtA family protein	Toxin
LOC22_RS15270 (LOC22_15270)	3207822..3208160	-	339	WP_196347930.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LOC22_RS15275 (LOC22_15275)	3208176..3208397	-	222	WP_196347931.1	DUF987 family protein	-
LOC22_RS15280 (LOC22_15280)	3208394..3208894	-	501	WP_050555520.1	DNA repair protein RadC	-
LOC22_RS15285 (LOC22_15285)	3208925..3209743	-	819	WP_196347933.1	DUF932 domain-containing protein	-
LOC22_RS15290 (LOC22_15290)	3209842..3210423	-	582	WP_230239016.1	hypothetical protein	-
LOC22_RS15295 (LOC22_15295)	3210806..3211699	-	894	WP_230239017.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (8-111)

Antitoxin

• Antitoxin CbeA (9-108)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14381.42 Da
• Isoelectric Point: 8.6199

>T225446 WP_196347929.1 NZ_CP087390:c3207786-3207403 [Enterobacter sp. AN-K1]
MQISTAPTTEPVSSRLSPVEVWQILLKHLLAQHYGLTLNDTPFSDESTIREHINAGISLSNAVNFIVEKYELVRTDHSGF
TIMEQSPFISSIDILRARRATGLMKRHGYKSISNITTGHLLQERHLR

Nucleotide

• Length: 384 bp

>T225446 NZ_CP121149:3895793-3896071 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12474.18 Da
• Isoelectric Point: 5.6016

>AT225446 WP_196347930.1 NZ_CP087390:c3208160-3207822 [Enterobacter sp. AN-K1]
MTKEPVQEHVWGLHSQTVPSFSARLVQEGRRLHFLADRATLLGTFSPEQSAALDAIFPALIRDMETALLSGKLDPREAKQ
YIYIRNGLTCESDTLGSCGYVYIVIYPQSEAS

Nucleotide

• Length: 339 bp

>AT225446 NZ_CP121149:3895530-3895790 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA