Detailed information of TA system TA225445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2873568..2874144
• Replicon: chromosome
• Accession: NZ_CP087390
• Organism: Enterobacter sp. AN-K1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: LOC22_RS13710
• Protein ID: WP_230238975.1
• Coordinates: 2873568..2873855 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: LOC22_RS13715
• Protein ID: WP_017694570.1
• Coordinates: 2873842..2874144 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOC22_RS13685 (LOC22_13685)	2868661..2869380	+	720	WP_017383867.1	winged helix-turn-helix domain-containing protein	-
LOC22_RS13690 (LOC22_13690)	2869530..2870000	+	471	WP_045333472.1	MarR family transcriptional regulator	-
LOC22_RS13695 (LOC22_13695)	2869997..2871064	+	1068	WP_230239553.1	HlyD family secretion protein	-
LOC22_RS13700 (LOC22_13700)	2871054..2872130	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
LOC22_RS13705 (LOC22_13705)	2872127..2873398	-	1272	WP_015572579.1	DUF445 domain-containing protein	-
LOC22_RS13710 (LOC22_13710)	2873568..2873855	+	288	WP_230238975.1	BrnT family toxin	Toxin
LOC22_RS13715 (LOC22_13715)	2873842..2874144	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
LOC22_RS13720 (LOC22_13720)	2874173..2874811	-	639	WP_017694569.1	LysE family translocator	-
LOC22_RS13725 (LOC22_13725)	2874850..2875602	-	753	WP_230238976.1	AraC family transcriptional regulator	-
LOC22_RS13730 (LOC22_13730)	2875756..2877126	+	1371	WP_017694568.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
LOC22_RS13735 (LOC22_13735)	2877304..2877849	-	546	WP_006810254.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11243.76 Da
• Isoelectric Point: 6.8624

>T225445 WP_230238975.1 NZ_CP087390:2873568-2873855 [Enterobacter sp. AN-K1]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVCFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T225445 NZ_CP121149:3658600-3659142 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT225445 WP_017694570.1 NZ_CP087390:2873842-2874144 [Enterobacter sp. AN-K1]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT225445 NZ_CP121149:3658305-3658616 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA