Detailed information of TA system TA225440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 347448..348059
• Replicon: chromosome
• Accession: NZ_CP087390
• Organism: Enterobacter sp. AN-K1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: LOC22_RS01645
• Protein ID: WP_012906750.1
• Coordinates: 347448..347627 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOC22_RS01650
• Protein ID: WP_045895535.1
• Coordinates: 347649..348059 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOC22_RS01625 (LOC22_01625)	343313..344188	+	876	WP_015570244.1	ATP-binding cassette domain-containing protein	-
LOC22_RS01630 (LOC22_01630)	344185..344901	+	717	WP_032619512.1	ABC transporter ATP-binding protein	-
LOC22_RS01635 (LOC22_01635)	345067..345537	+	471	WP_017384432.1	hypothetical protein	-
LOC22_RS01640 (LOC22_01640)	345543..346469	-	927	WP_017384433.1	LysR substrate-binding domain-containing protein	-
LOC22_RS01645 (LOC22_01645)	347448..347627	+	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOC22_RS01650 (LOC22_01650)	347649..348059	+	411	WP_045895535.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOC22_RS01655 (LOC22_01655)	348141..348707	+	567	WP_230239290.1	serine acetyltransferase	-
LOC22_RS01660 (LOC22_01660)	348711..349133	-	423	WP_230239291.1	tail fiber assembly protein	-
LOC22_RS01665 (LOC22_01665)	349135..351696	-	2562	WP_230239292.1	phage tail protein	-
LOC22_RS01670 (LOC22_01670)	351699..352250	-	552	WP_230239293.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fliA	344197..397744	53547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T225440 WP_012906750.1 NZ_CP087390:347448-347627 [Enterobacter sp. AN-K1]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T225440 NZ_CP121149:c2613165-2612989 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14904.82 Da
• Isoelectric Point: 4.3170

>AT225440 WP_045895535.1 NZ_CP087390:347649-348059 [Enterobacter sp. AN-K1]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDDYQDAIESVREAIEAHIELLVEDGETVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Nucleotide

• Length: 411 bp

>AT225440 NZ_CP121149:c2612943-2612527 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4