Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1292597..1293119 Replicon chromosome
Accession NZ_CP087379
Organism Mannheimia haemolytica strain IMT47952

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag LO774_RS06555 Protein ID WP_006249269.1
Coordinates 1292597..1292893 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A249A190
Locus tag LO774_RS06560 Protein ID WP_006249270.1
Coordinates 1292877..1293119 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO774_RS06540 (LO774_06540) 1287651..1288976 + 1326 WP_020831251.1 anti-phage deoxyguanosine triphosphatase -
LO774_RS06545 (LO774_06545) 1289097..1289396 + 300 WP_006249265.1 hypothetical protein -
LO774_RS06550 (LO774_06550) 1289558..1292470 + 2913 WP_006249267.1 RNA polymerase-associated protein RapA -
LO774_RS06555 (LO774_06555) 1292597..1292893 + 297 WP_006249269.1 BrnT family toxin Toxin
LO774_RS06560 (LO774_06560) 1292877..1293119 + 243 WP_006249270.1 CopG family antitoxin Antitoxin
LO774_RS06565 (LO774_06565) 1293131..1293799 + 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
LO774_RS06570 (LO774_06570) 1293777..1294352 - 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
LO774_RS06575 (LO774_06575) 1294412..1295254 + 843 WP_006250575.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
LO774_RS06580 (LO774_06580) 1295432..1296487 - 1056 WP_006251368.1 tyrosine-type recombinase/integrase -
LO774_RS06585 (LO774_06585) 1296447..1296722 - 276 WP_006248810.1 DUF4224 domain-containing protein -
LO774_RS06590 (LO774_06590) 1296749..1297240 - 492 WP_021279670.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1289097..1344463 55366


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T225426 WP_006249269.1 NZ_CP087379:1292597-1292893 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T225426 NZ_CP121147:c4286344-4285994 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATTTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9346.79 Da        Isoelectric Point: 7.1665

>AT225426 WP_006249270.1 NZ_CP087379:1292877-1293119 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT225426 NZ_CP121147:c4286589-4286338 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A249A190

References