Detailed information of TA system TA225425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 987949..988471
• Replicon: chromosome
• Accession: NZ_CP087379
• Organism: Mannheimia haemolytica strain IMT47952

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LO774_RS05065
• Protein ID: WP_040080572.1
• Coordinates: 988202..988471 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A248ZY54
• Locus tag: LO774_RS05060
• Protein ID: WP_006250323.1
• Coordinates: 987949..988215 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO774_RS05040 (LO774_05040)	983432..984472	-	1041	WP_230269058.1	IS481 family transposase	-
LO774_RS05045 (LO774_05045)	984810..986195	-	1386	WP_006251981.1	GTPase HflX	-
LO774_RS05050 (LO774_05050)	986215..986487	-	273	WP_006250326.1	RNA chaperone Hfq	-
LO774_RS05055 (LO774_05055)	986556..987509	-	954	WP_040080573.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
LO774_RS05060 (LO774_05060)	987949..988215	+	267	WP_006250323.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LO774_RS05065 (LO774_05065)	988202..988471	+	270	WP_040080572.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
LO774_RS05070 (LO774_05070)	988490..990373	-	1884	WP_061887196.1	DNA mismatch repair endonuclease MutL	-
LO774_RS05075 (LO774_05075)	990657..991895	+	1239	WP_061887197.1	glutamate-5-semialdehyde dehydrogenase	-
LO774_RS05080 (LO774_05080)	991897..992742	+	846	WP_061887198.1	virulence factor BrkB family protein	-
LO774_RS05085 (LO774_05085)	992717..993151	+	435	WP_006250318.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10508.09 Da
• Isoelectric Point: 6.4732

>T225425 WP_040080572.1 NZ_CP087379:988202-988471 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVEFLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Nucleotide

• Length: 270 bp

>T225425 NZ_CP121147:c4274940-4274650 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9738.10 Da
• Isoelectric Point: 4.7954

>AT225425 WP_006250323.1 NZ_CP087379:987949-988215 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Nucleotide

• Length: 267 bp

>AT225425 NZ_CP121147:c4275172-4274930 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A248ZY54