Detailed information of TA system TA225423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 613857..614572
• Replicon: chromosome
• Accession: NZ_CP087379
• Organism: Mannheimia haemolytica strain IMT47952

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LO774_RS03085
• Protein ID: WP_006250491.1
• Coordinates: 613857..614285 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248ZW87
• Locus tag: LO774_RS03090
• Protein ID: WP_006248272.1
• Coordinates: 614285..614572 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO774_RS03070 (LO774_03070)	609112..610764	+	1653	WP_040080487.1	phospho-sugar mutase	-
LO774_RS03075 (LO774_03075)	610817..611750	+	934	Protein_593	nucleoside hydrolase	-
LO774_RS03080 (LO774_03080)	611772..613853	-	2082	WP_040080489.1	ATP-dependent DNA helicase RecG	-
LO774_RS03085 (LO774_03085)	613857..614285	-	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
LO774_RS03090 (LO774_03090)	614285..614572	-	288	WP_006248272.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LO774_RS03095 (LO774_03095)	614736..616619	+	1884	WP_040080490.1	molecular chaperone HtpG	-
LO774_RS03100 (LO774_03100)	616708..616854	+	147	WP_230269068.1	hypothetical protein	-
LO774_RS03105 (LO774_03105)	616999..618039	+	1041	WP_230269069.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	616999..618039	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T225423 WP_006250491.1 NZ_CP087379:c614285-613857 [Mannheimia haemolytica]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T225423 NZ_CP121147:3678604-3679146 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTATTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTAATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11085.39 Da
• Isoelectric Point: 4.3648

>AT225423 WP_006248272.1 NZ_CP087379:c614572-614285 [Mannheimia haemolytica]
MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT225423 NZ_CP121147:3678309-3678620 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A248ZW87