Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 102483..103133 Replicon chromosome
Accession NZ_CP087379
Organism Mannheimia haemolytica strain IMT47952

Toxin (Protein)


Gene name vapC Uniprot ID A0A547EDJ6
Locus tag LO774_RS00555 Protein ID WP_006249411.1
Coordinates 102483..102872 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D2Q6P1
Locus tag LO774_RS00560 Protein ID WP_006251766.1
Coordinates 102873..103133 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO774_RS00510 (LO774_00510) 97963..98772 - 810 WP_006250123.1 metal ABC transporter permease -
LO774_RS00515 (LO774_00515) 98765..99625 - 861 WP_061887112.1 metal ABC transporter permease -
LO774_RS00520 (LO774_00520) 99699..100484 - 786 WP_061887113.1 GDP-L-fucose synthase -
LO774_RS00550 (LO774_00550) 101225..102463 - 1239 WP_006251767.1 peptidase T -
LO774_RS00555 (LO774_00555) 102483..102872 - 390 WP_006249411.1 type II toxin-antitoxin system VapC family toxin Toxin
LO774_RS00560 (LO774_00560) 102873..103133 - 261 WP_006251766.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LO774_RS00565 (LO774_00565) 103249..103461 - 213 WP_006251765.1 BrnA antitoxin family protein -
LO774_RS00570 (LO774_00570) 103462..103749 - 288 WP_006251764.1 BrnT family toxin -
LO774_RS00575 (LO774_00575) 103912..107445 - 3534 WP_006251763.1 transcription-repair coupling factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14633.91 Da        Isoelectric Point: 7.1041

>T225422 WP_006249411.1 NZ_CP087379:c102872-102483 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T225422 NZ_CP121147:3644619-3644837 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.50 Da        Isoelectric Point: 4.6909

>AT225422 WP_006251766.1 NZ_CP087379:c103133-102873 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT225422 NZ_CP121147:3644219-3644593 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A547EDJ6

Antitoxin

Source ID Structure
AlphaFold DB A0A1D2Q6P1

References