Detailed information of TA system TA225418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 5172..5731
• Replicon: plasmid pRIVM_C019217_2
• Accession: NZ_CP087378
• Organism: Escherichia coli strain RIVM_C019217

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G4VUF8
• Locus tag: LN359_RS25405
• Protein ID: WP_000421273.1
• Coordinates: 5172..5447 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: LN359_RS25410
• Protein ID: WP_001387467.1
• Coordinates: 5447..5731 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN359_RS25370 (1221)	1221..1961	-	741	WP_001066941.1	tyrosine-type recombinase/integrase	-
LN359_RS25375 (2082)	2082..2264	-	183	WP_042344623.1	hypothetical protein	-
LN359_RS25380 (2331)	2331..2621	-	291	Protein_3	type II toxin-antitoxin system RelE/ParE family toxin	-
LN359_RS25385 (2608)	2608..2876	-	269	Protein_4	type II toxin-antitoxin system ParD family antitoxin	-
LN359_RS25390 (3408)	3408..4577	+	1170	WP_001403369.1	hypothetical protein	-
LN359_RS25395 (4610)	4610..4738	+	129	Protein_6	IS481 family transposase	-
LN359_RS25400 (4863)	4863..5066	-	204	Protein_7	hypothetical protein	-
LN359_RS25405 (5172)	5172..5447	-	276	WP_000421273.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LN359_RS25410 (5447)	5447..5731	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
LN359_RS25415 (6336)	6336..7088	+	753	WP_001403368.1	IclR family transcriptional regulator	-
LN359_RS25420 (7134)	7134..8099	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
LN359_RS25425 (8132)	8132..8512	-	381	WP_000710783.1	Rid family detoxifying hydrolase	-
LN359_RS25430 (8537)	8537..9427	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
LN359_RS25435 (9780)	9780..10346	+	567	Protein_14	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	-	1..147403	147403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10765.45 Da
• Isoelectric Point: 9.9324

>T225418 WP_000421273.1 NZ_CP087378:c5447-5172 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T225418 NZ_CP121147:c2394276-2393998 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCAGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT225418 WP_001387467.1 NZ_CP087378:c5731-5447 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT225418 NZ_CP121147:c2393998-2393714 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6H1

Antitoxin

• AlphaFold DB: A0A0E0Y880