Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4731864..4732361 Replicon chromosome
Accession NZ_CP087377
Organism Escherichia coli strain RIVM_C019217

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LN359_RS23085 Protein ID WP_001173343.1
Coordinates 4731864..4732037 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LN359_RS23090 Protein ID WP_000398619.1
Coordinates 4732065..4732361 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN359_RS23075 (4728534) 4728534..4729991 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
LN359_RS23080 (4730228) 4730228..4731745 + 1518 WP_001550472.1 lysine--tRNA ligase -
LN359_RS23085 (4731864) 4731864..4732037 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LN359_RS23090 (4732065) 4732065..4732361 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LN359_RS23095 (4732590) 4732590..4732862 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LN359_RS23100 (4732874) 4732874..4733104 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
LN359_RS23105 (4733284) 4733284..4734915 + 1632 WP_001216477.1 sensor histidine kinase -
LN359_RS23110 (4734912) 4734912..4735631 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T225416 WP_001173343.1 NZ_CP087377:c4732037-4731864 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T225416 NZ_CP121147:c2154527-2154420 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT225416 WP_000398619.1 NZ_CP087377:c4732361-4732065 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT225416 NZ_CP121147:2154580-2154641 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References