Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3923477..3924095 Replicon chromosome
Accession NZ_CP087377
Organism Escherichia coli strain RIVM_C019217

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LN359_RS19195 Protein ID WP_001291435.1
Coordinates 3923877..3924095 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LN359_RS19190 Protein ID WP_000344800.1
Coordinates 3923477..3923851 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN359_RS19180 (3918566) 3918566..3919759 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LN359_RS19185 (3919782) 3919782..3922931 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LN359_RS19190 (3923477) 3923477..3923851 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LN359_RS19195 (3923877) 3923877..3924095 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LN359_RS19200 (3924267) 3924267..3924818 + 552 WP_000102564.1 maltose O-acetyltransferase -
LN359_RS19205 (3924934) 3924934..3925404 + 471 WP_000136192.1 YlaC family protein -
LN359_RS19210 (3925568) 3925568..3927118 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LN359_RS19215 (3927160) 3927160..3927513 - 354 WP_085334692.1 DUF1428 family protein -
LN359_RS19225 (3927892) 3927892..3928203 + 312 WP_000409911.1 MGMT family protein -
LN359_RS19230 (3928234) 3928234..3928806 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225411 WP_001291435.1 NZ_CP087377:3923877-3924095 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T225411 NZ_CP121147:c878923-878549 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT225411 WP_000344800.1 NZ_CP087377:3923477-3923851 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT225411 NZ_CP121147:c879381-879013 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCACGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References