Detailed information of TA system TA225410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3470430..3471135
• Replicon: chromosome
• Accession: NZ_CP087377
• Organism: Escherichia coli strain RIVM_C019217

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LN359_RS17080
• Protein ID: WP_000539521.1
• Coordinates: 3470430..3470816 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LN359_RS17085
• Protein ID: WP_001280945.1
• Coordinates: 3470806..3471135 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN359_RS17060 (3466434)	3466434..3467060	+	627	WP_001295292.1	glutathione S-transferase GstB	-
LN359_RS17065 (3467057)	3467057..3468172	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
LN359_RS17070 (3468283)	3468283..3468666	-	384	WP_000497137.1	biofilm formation regulator BssR	-
LN359_RS17075 (3468879)	3468879..3470204	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LN359_RS17080 (3470430)	3470430..3470816	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LN359_RS17085 (3470806)	3470806..3471135	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LN359_RS17090 (3471205)	3471205..3472533	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
LN359_RS17095 (3472541)	3472541..3474889	-	2349	WP_001550886.1	EAL domain-containing protein	-
LN359_RS17100 (3475067)	3475067..3475978	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T225410 WP_000539521.1 NZ_CP087377:3470430-3470816 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T225410 NZ_CP121147:764875-765171 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT225410 WP_001280945.1 NZ_CP087377:3470806-3471135 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT225410 NZ_CP121147:765173-765568 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6