Detailed information of TA system TA225408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2838397..2839035
• Replicon: chromosome
• Accession: NZ_CP087377
• Organism: Escherichia coli strain RIVM_C019217

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: LN359_RS13920
• Protein ID: WP_000813795.1
• Coordinates: 2838859..2839035 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LN359_RS13915
• Protein ID: WP_076797675.1
• Coordinates: 2838397..2838813 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN359_RS13895 (2833549)	2833549..2834490	-	942	WP_001251315.1	ABC transporter permease	-
LN359_RS13900 (2834491)	2834491..2835504	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
LN359_RS13905 (2835522)	2835522..2836667	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
LN359_RS13910 (2836912)	2836912..2838318	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
LN359_RS13915 (2838397)	2838397..2838813	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LN359_RS13920 (2838859)	2838859..2839035	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LN359_RS13925 (2839257)	2839257..2839487	+	231	WP_023910283.1	YncJ family protein	-
LN359_RS13930 (2839579)	2839579..2841540	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LN359_RS13935 (2841613)	2841613..2842149	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
LN359_RS13940 (2842241)	2842241..2843413	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T225408 WP_000813795.1 NZ_CP087377:c2839035-2838859 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T225408 NZ_CP121147:325437-325712 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT225408 WP_076797675.1 NZ_CP087377:c2838813-2838397 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT225408 NZ_CP121147:325709-326068 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1