Detailed information of TA system TA022540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 117493..118139
• Replicon: plasmid pBMUL01
• Accession: NC_010070
• Organism: Burkholderia multivorans ATCC 17616

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BMUL_RS31490
• Protein ID: WP_012211060.1
• Coordinates: 117732..118139 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BMUL_RS34295
• Protein ID: WP_009692911.1
• Coordinates: 117493..117735 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMUL_RS31470	112628..113485	-	858	WP_012211056.1	replication initiator protein A	-
BMUL_RS31475	114230..115195	-	966	WP_012211057.1	ParB/RepB/Spo0J family partition protein	-
BMUL_RS31480	115195..115866	-	672	WP_012211058.1	AAA family ATPase	-
BMUL_RS31485	116360..117391	+	1032	WP_012211059.1	non-homologous end-joining DNA ligase	-
BMUL_RS34295	117493..117735	+	243	WP_009692911.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BMUL_RS31490	117732..118139	+	408	WP_012211060.1	type II toxin-antitoxin system VapC family toxin	Toxin
BMUL_RS31495	118483..118803	+	321	WP_012211061.1	hypothetical protein	-
BMUL_RS31500	118835..119443	+	609	WP_012211062.1	hypothetical protein	-
BMUL_RS35125	119440..119598	+	159	WP_009692916.1	hypothetical protein	-
BMUL_RS31505	119591..120511	+	921	WP_009692917.1	serine protease	-
BMUL_RS31510	120623..120808	+	186	WP_009692918.1	hypothetical protein	-
BMUL_RS31515	120819..121127	+	309	WP_009692919.1	hypothetical protein	-
BMUL_RS31520	121124..121855	+	732	WP_009692920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..167422	167422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (12-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14613.77 Da
• Isoelectric Point: 5.1402

>T22540 WP_012211060.1 NC_010070:117732-118139 [Burkholderia multivorans ATCC 17616]
MSLYLLDTNILSNVIRDPRGACASRIGETRPEQVCTSIVVAAELRYGVCKRGSSALAQRVEQLLASLTVLPLQSDADQCY
GRLRADLERQGQLIGANDMLIAAHAMAVDAVLVTDNTAEFTRVTGLAVENWLRPA

Nucleotide

• Length: 408 bp

>T22540 NC_010070:117732-118139 [Burkholderia multivorans ATCC 17616]
ATGAGCCTCTATCTGCTCGACACCAATATTTTGTCGAACGTGATCCGAGACCCACGGGGCGCATGCGCTTCGCGCATCGG
GGAAACGCGGCCCGAGCAAGTGTGCACCTCGATCGTCGTCGCGGCCGAGCTACGGTACGGCGTCTGTAAGCGCGGCTCCT
CGGCGCTCGCCCAGCGTGTCGAGCAGCTGCTCGCGAGCTTGACCGTATTACCGCTGCAGTCCGACGCGGATCAGTGCTAC
GGGCGGCTGCGAGCCGATCTCGAGAGGCAGGGTCAGTTGATCGGGGCGAACGACATGCTGATCGCTGCCCACGCGATGGC
CGTCGACGCCGTCTTGGTCACGGACAATACGGCCGAATTCACTCGCGTTACAGGACTCGCCGTCGAAAACTGGCTCCGGC
CTGCGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9309.54 Da
• Isoelectric Point: 4.8615

>AT22540 WP_009692911.1 NC_010070:117493-117735 [Burkholderia multivorans ATCC 17616]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAPSLRELFAQWAPLDETFPDIENLPAEPVDIE

Nucleotide

• Length: 243 bp

>AT22540 NC_010070:117493-117735 [Burkholderia multivorans ATCC 17616]
ATGCCAACCCATGACACCCGCCACGCACGCCTATTTCGCAACGGACGTAGCCAGGCCGTGCGGATCCCGCGCGAGTTCGA
GCTGCCGGTCTCGGAAGTCACTATTCATCGTGAAGGCCGGCGCCTAATCATCGAACCGGTCGAAGCGCCGTCTCTACGCG
AGCTATTTGCGCAATGGGCGCCCTTGGACGAAACTTTCCCTGACATCGAAAACCTCCCCGCTGAACCGGTCGATATTGAA
TGA