Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 957597..958392 Replicon chromosome
Accession NZ_CP087377
Organism Escherichia coli strain RIVM_C019217

Toxin (Protein)


Gene name yeeV Uniprot ID A0A4Y9BN12
Locus tag LN359_RS04650 Protein ID WP_023909482.1
Coordinates 957597..957971 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A4Y9BHM8
Locus tag LN359_RS04655 Protein ID WP_023909483.1
Coordinates 958018..958392 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN359_RS04620 (953562) 953562..954827 - 1266 WP_001218869.1 integrase arm-type DNA-binding domain-containing protein -
LN359_RS04625 (955291) 955291..955617 - 327 WP_001522265.1 type II toxin-antitoxin system RelE/ParE family toxin -
LN359_RS04630 (955614) 955614..955877 - 264 WP_001143297.1 type II toxin-antitoxin system ParD family antitoxin -
LN359_RS04635 (955949) 955949..956815 - 867 WP_023909479.1 DUF4942 domain-containing protein -
LN359_RS04640 (956900) 956900..957097 - 198 WP_001522268.1 DUF957 domain-containing protein -
LN359_RS04645 (957109) 957109..957600 - 492 WP_023909481.1 DUF5983 family protein -
LN359_RS04650 (957597) 957597..957971 - 375 WP_023909482.1 TA system toxin CbtA family protein Toxin
LN359_RS04655 (958018) 958018..958392 - 375 WP_023909483.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
LN359_RS04660 (958472) 958472..958693 - 222 WP_023909484.1 DUF987 domain-containing protein -
LN359_RS04665 (958745) 958745..959500 - 756 WP_001522280.1 IS21-like element ISEc10 family helper ATPase IstB -
LN359_RS04670 (959497) 959497..960996 - 1500 WP_059332257.1 IS21-like element ISEc10 family transposase -
LN359_RS04675 (961177) 961177..961653 - 477 WP_001186724.1 RadC family protein -
LN359_RS04680 (961668) 961668..962141 - 474 WP_001522284.1 antirestriction protein -
LN359_RS04685 (962236) 962236..962412 - 177 WP_001522285.1 hypothetical protein -
LN359_RS04690 (962412) 962412..963230 - 819 WP_001522287.1 DUF932 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX 765876..960920 195044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(19-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14009.98 Da        Isoelectric Point: 8.2905

>T225395 WP_023909482.1 NZ_CP087377:c957971-957597 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK

Download         Length: 375 bp

>T225395 NZ_CP121145:563545-563829 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTTAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13598.31 Da        Isoelectric Point: 5.5051

>AT225395 WP_023909483.1 NZ_CP087377:c958392-958018 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT225395 NZ_CP121145:563313-563555 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCTGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A4Y9BN12


Antitoxin

Source ID Structure
AlphaFold DB A0A4Y9BHM8

References