225387

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 5282..5870 Replicon plasmid p2DB003
Accession NZ_CP087353
Organism Acinetobacter baumannii strain DB003

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag LO008_RS20335 Protein ID WP_000438826.1
Coordinates 5282..5569 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9M5I3
Locus tag LO008_RS20340 Protein ID WP_001983304.1
Coordinates 5556..5870 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO008_RS20310 1422..2372 + 951 WP_001205343.1 replication initiation protein RepM -
LO008_RS20315 2365..2940 + 576 WP_001096616.1 plasmid replication DNA-binding protein -
LO008_RS20320 2960..3103 + 144 WP_001125246.1 hypothetical protein -
LO008_RS20325 3286..4113 - 828 WP_000713530.1 OXA-24 family carbapenem-hydrolyzing class D beta-lactamase OXA-72 -
LO008_RS20330 4265..5011 + 747 WP_005262957.1 IS5-like element ISAba31 family transposase -
LO008_RS20335 5282..5569 + 288 WP_000438826.1 BrnT family toxin Toxin
LO008_RS20340 5556..5870 + 315 WP_001983304.1 BrnA antitoxin family protein Antitoxin
LO008_RS20345 5998..6912 + 915 Protein_8 TonB-dependent receptor ZnuD2 -
LO008_RS20350 6996..7988 + 993 WP_005028482.1 IS5-like element ISAba23 family transposase -
LO008_RS20355 8161..9663 + 1503 Protein_10 TonB-dependent receptor ZnuD2 -
LO008_RS20360 9968..10429 + 462 WP_000761346.1 DIP1984 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-72 - 1..11084 11084
- inside IScluster/Tn blaOXA-72 - 3286..7988 4702


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T225387 WP_000438826.1 NZ_CP087353:5282-5569 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T225387 NZ_CP121143:c4025418-4025035 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12208.84 Da        Isoelectric Point: 7.2026

>AT225387 WP_001983304.1 NZ_CP087353:5556-5870 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT225387 NZ_CP121143:c4025660-4025418 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB N9M5I3

References