Detailed information of TA system TA225378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 65227..65815
• Replicon: plasmid p3DB008
• Accession: NZ_CP087341
• Organism: Acinetobacter baumannii strain DB008

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5K1MH32
• Locus tag: LO011_RS18820
• Protein ID: WP_000569859.1
• Coordinates: 65519..65815 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LO011_RS18815
• Protein ID: WP_000241337.1
• Coordinates: 65227..65517 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO011_RS18780	61042..62154	-	1113	WP_000842149.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
LO011_RS18785	62176..62451	-	276	WP_001133596.1	metal-sensing transcriptional repressor	-
LO011_RS18790	62579..62668	+	90	Protein_63	transcriptional regulator	-
LO011_RS18795	62695..63027	-	333	WP_001128380.1	zinc ribbon domain-containing protein	-
LO011_RS18800	63264..64496	-	1233	Protein_65	IS3 family transposase	-
LO011_RS18805	64548..64709	-	162	Protein_66	transposase	-
LO011_RS18810	64776..64947	+	172	Protein_67	IS5/IS1182 family transposase	-
LO011_RS18815	65227..65517	-	291	WP_000241337.1	putative addiction module antidote protein	Antitoxin
LO011_RS18820	65519..65815	-	297	WP_000569859.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO011_RS18825	66441..66935	+	495	Protein_70	IS5 family transposase	-
LO011_RS18830	66973..67125	-	153	WP_162900102.1	hypothetical protein	-
LO011_RS18840	69103..69498	-	396	Protein_73	transposase	-
LO011_RS18845	69533..69937	-	405	WP_005134208.1	transposase family protein	-
LO011_RS18850	70054..70279	-	226	Protein_75	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93962	93962
	-	inside	IScluster/Tn	-	-	63264..68467	5203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11554.35 Da
• Isoelectric Point: 8.5037

>T225378 WP_000569859.1 NZ_CP087341:c65815-65519 [Acinetobacter baumannii]
MIEIKRLPEFDEWLDGLKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGAGWRMYYIQHGDVVIVMLGGGEKS
TQKDDIKKAVKLSKTLED

Nucleotide

• Length: 297 bp

>T225378 NZ_CP121141:3844314-3845636 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGTAATATCACTG
CCGACGCGGTGTATCACTACTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCATGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGAGAGCAGATGTTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10584.24 Da
• Isoelectric Point: 5.1133

>AT225378 WP_000241337.1 NZ_CP087341:c65517-65227 [Acinetobacter baumannii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSTPRFDTI
NRVVNALGLKLTVQHT

Nucleotide

• Length: 291 bp

>AT225378 NZ_CP121141:3844048-3844314 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A5K1MH32