Detailed information of TA system TA225367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 37025..37613
• Replicon: plasmid p1OC074
• Accession: NZ_CP087329
• Organism: Acinetobacter baumannii strain OC074

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5K1MH32
• Locus tag: LOZ98_RS19135
• Protein ID: WP_000569859.1
• Coordinates: 37025..37321 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LOZ98_RS19140
• Protein ID: WP_000241337.1
• Coordinates: 37323..37613 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ98_RS19120	32999..34291	+	1293	WP_024436605.1	MFS transporter	-
LOZ98_RS19125	34304..35209	+	906	WP_005130397.1	aromatic amino acid DMT transporter YddG	-
LOZ98_RS19130	35354..36688	+	1335	WP_032050497.1	ISNCY family transposase	-
LOZ98_RS19135	37025..37321	+	297	WP_000569859.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOZ98_RS19140	37323..37613	+	291	WP_000241337.1	putative addiction module antidote protein	Antitoxin
LOZ98_RS19145	37975..39140	+	1166	WP_123794023.1	IS3-like element ISAba22 family transposase	-
LOZ98_RS19150	39274..40497	+	1224	WP_123794025.1	IS256 family transposase	-
LOZ98_RS19155	40561..40701	-	141	Protein_45	antibiotic biosynthesis monooxygenase	-
LOZ98_RS19160	40834..41109	+	276	WP_001133596.1	metal-sensing transcriptional repressor	-
LOZ98_RS19165	41131..42243	+	1113	WP_000842149.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..88290	88290
	-	inside	IScluster/Tn	-	-	27570..40497	12927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11554.35 Da
• Isoelectric Point: 8.5037

>T225367 WP_000569859.1 NZ_CP087329:37025-37321 [Acinetobacter baumannii]
MIEIKRLPEFDEWLDGLKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGAGWRMYYIQHGDVVIVMLGGGEKS
TQKDDIKKAVKLSKTLED

Nucleotide

• Length: 297 bp

>T225367 NZ_CP121139:5103130-5103513 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10584.24 Da
• Isoelectric Point: 5.1133

>AT225367 WP_000241337.1 NZ_CP087329:37323-37613 [Acinetobacter baumannii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSTPRFDTI
NRVVNALGLKLTVQHT

Nucleotide

• Length: 291 bp

>AT225367 NZ_CP121139:5102888-5103130 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A5K1MH32