Detailed information of TA system TA225365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2658709..2660450
• Replicon: chromosome
• Accession: NZ_CP087328
• Organism: Acinetobacter baumannii strain OC074

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: N9S537
• Locus tag: LOZ98_RS13225
• Protein ID: WP_000233088.1
• Coordinates: 2659794..2660450 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: N9RUZ1
• Locus tag: LOZ98_RS13220
• Protein ID: WP_004669001.1
• Coordinates: 2658709..2659794 (-)
• Length: 362 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOZ98_RS13205	2655673..2656236	-	564	WP_001133235.1	TetR/AcrR family transcriptional regulator	-
LOZ98_RS13210	2656283..2656423	+	141	WP_000504068.1	hypothetical protein	-
LOZ98_RS13215	2657041..2657919	-	879	WP_000732870.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
LOZ98_RS13220	2658709..2659794	-	1086	WP_004669001.1	macro domain-containing protein	Antitoxin
LOZ98_RS13225	2659794..2660450	-	657	WP_000233088.1	DUF4433 domain-containing protein	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (18-218)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25316.92 Da
• Isoelectric Point: 7.1033

>T225365 WP_000233088.1 NZ_CP087328:c2660450-2659794 [Acinetobacter baumannii]
MVGRNYSDVLNPQKALIWRIVHRDNIPWILENGLHSSNSPTQCVNWVQIGSPELIEKRQLHPVPVGKKGVLSDYIPFYFT
PFSPMLLNIKSGRGGVRQRTNDEIVILVSSLYQLQQQQIPFVFTNSHAYYQWAEFYTDLSSLNEIDWSILQARDFKRDMD
DPAKFERYQAEALVHQHCPTKVLIGVICYTETVKAQIEQWLQQADVNIPVHVRQGVYF

Nucleotide

• Length: 657 bp

>T225365 NZ_CP121139:c4698638-4698270 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 362 a.a.
• Molecular weight: 41015.11 Da
• Isoelectric Point: 5.8156

>AT225365 WP_004669001.1 NZ_CP087328:c2659794-2658709 [Acinetobacter baumannii]
MIRYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPKNMAAYSQACKAGQVTTGKMFVTETNELMGARWIINFPTK
QHWRDKSKLQWIEDGLQDLKKFIIDHQVKSIAIPPLGAGNGGLEWSRVKSKIETELSDLEYVDILIFEPTTQYQNVAKKI
GVSQLTPARAMIAELVRRYWVLGIECSLLEIQKLAWFLQRVIEQKQLPNELKLNFQANYYGPYASNLDHLLNAMDGSYLK
SDKRIPDCDPLDVIWFNDQQKSKVQGYLISEAKEYMAAVEGAATLIEGFESPFGMELLATVDWLIYKEGYQPNVESIQEG
LANWSAGERWARRKSDLFKAEHIQFALQKLDGFAYSYSDVT

Nucleotide

• Length: 1086 bp

>AT225365 NZ_CP121139:c4698856-4698635 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: N9S537

Antitoxin

• AlphaFold DB: N9RUZ1