Detailed information of TA system TA225361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 88161..88749
• Replicon: plasmid p1OC073
• Accession: NZ_CP087326
• Organism: Acinetobacter baumannii strain OC073

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5K1MH32
• Locus tag: LO013_RS18775
• Protein ID: WP_000569859.1
• Coordinates: 88161..88457 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LO013_RS18780
• Protein ID: WP_000241337.1
• Coordinates: 88459..88749 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO013_RS18740	83623..83979	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LO013_RS18745	83972..84274	+	303	WP_024436604.1	XRE family transcriptional regulator	-
LO013_RS18750	84267..84476	+	210	WP_000069475.1	hypothetical protein	-
LO013_RS18755	84651..85169	+	519	WP_000447196.1	tetratricopeptide repeat protein	-
LO013_RS18760	85208..85720	+	513	WP_024436968.1	SMI1/KNR4 family protein	-
LO013_RS18765	85903..86331	+	429	WP_016653412.1	hypothetical protein	-
LO013_RS18770	86490..87824	+	1335	WP_032050497.1	ISNCY family transposase	-
LO013_RS18775	88161..88457	+	297	WP_000569859.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO013_RS18780	88459..88749	+	291	WP_000241337.1	putative addiction module antidote protein	Antitoxin
LO013_RS18785	89111..90276	+	1166	WP_123794023.1	IS3-like element ISAba22 family transposase	-
LO013_RS18790	90410..91633	+	1224	WP_123794025.1	IS256 family transposase	-
LO013_RS18795	91697..91981	-	285	WP_000156168.1	putative quinol monooxygenase	-
LO013_RS18800	92002..93000	-	999	WP_004740250.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113509	113509
	-	inside	IScluster/Tn	-	-	86502..98478	11976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11554.35 Da
• Isoelectric Point: 8.5037

>T225361 WP_000569859.1 NZ_CP087326:88161-88457 [Acinetobacter baumannii]
MIEIKRLPEFDEWLDGLKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGAGWRMYYIQHGDVVIVMLGGGEKS
TQKDDIKKAVKLSKTLED

Nucleotide

• Length: 297 bp

>T225361 NZ_CP121139:2333516-2334838 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGTAATATCACTG
CCGACGCGGTGTATCACTACTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCATGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGAGAGCAGATGTTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10584.24 Da
• Isoelectric Point: 5.1133

>AT225361 WP_000241337.1 NZ_CP087326:88459-88749 [Acinetobacter baumannii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSTPRFDTI
NRVVNALGLKLTVQHT

Nucleotide

• Length: 291 bp

>AT225361 NZ_CP121139:2333250-2333516 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A5K1MH32