225349

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 8933..9521 Replicon plasmid p1OC064
Accession NZ_CP087319
Organism Acinetobacter baumannii strain OC064

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag LO012_RS20095 Protein ID WP_000438826.1
Coordinates 9234..9521 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9M5I3
Locus tag LO012_RS20090 Protein ID WP_001983304.1
Coordinates 8933..9247 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO012_RS20070 4761..5132 + 372 WP_000504218.1 hypothetical protein -
LO012_RS20075 5132..5305 + 174 WP_001282484.1 hypothetical protein -
LO012_RS20080 5628..6089 - 462 WP_000761346.1 DIP1984 family protein -
LO012_RS20085 6394..8805 - 2412 WP_000932953.1 TonB-dependent receptor ZnuD2 -
LO012_RS20090 8933..9247 - 315 WP_001983304.1 BrnA antitoxin family protein Antitoxin
LO012_RS20095 9234..9521 - 288 WP_000438826.1 BrnT family toxin Toxin
LO012_RS20100 9827..10078 + 252 Protein_12 hypothetical protein -
LO012_RS20105 10130..10834 - 705 WP_001067856.1 IS6-like element IS26 family transposase -
LO012_RS20110 10964..11779 + 816 WP_000018326.1 aminoglycoside O-phosphotransferase APH(3')-Ia -
LO012_RS20115 11933..12112 - 180 WP_000902128.1 hypothetical protein -
LO012_RS20120 12204..12908 - 705 WP_001067856.1 IS6-like element IS26 family transposase -
LO012_RS20125 13038..13853 + 816 WP_000018326.1 aminoglycoside O-phosphotransferase APH(3')-Ia -
LO012_RS20130 14007..14186 - 180 WP_000902128.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / blaOXA-58 - 1..36555 36555


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T225349 WP_000438826.1 NZ_CP087319:c9521-9234 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T225349 NZ_CP121137:c3543443-3543159 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTTAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12208.84 Da        Isoelectric Point: 7.2026

>AT225349 WP_001983304.1 NZ_CP087319:c9247-8933 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT225349 NZ_CP121137:c3543675-3543433 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCTGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB N9M5I3

References