Detailed information of TA system TA225348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 30885..31473
• Replicon: plasmid p2OC064
• Accession: NZ_CP087318
• Organism: Acinetobacter baumannii strain OC064

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: LO012_RS19990
• Protein ID: WP_000438826.1
• Coordinates: 31186..31473 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: LO012_RS19985
• Protein ID: WP_001983304.1
• Coordinates: 30885..31199 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO012_RS19965	26713..27084	+	372	WP_000504218.1	hypothetical protein	-
LO012_RS19970	27084..27257	+	174	WP_001282484.1	hypothetical protein	-
LO012_RS19975	27580..28041	-	462	WP_000761346.1	DIP1984 family protein	-
LO012_RS19980	28346..30757	-	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
LO012_RS19985	30885..31199	-	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
LO012_RS19990	31186..31473	-	288	WP_000438826.1	BrnT family toxin	Toxin
LO012_RS19995	31779..32030	+	252	Protein_36	hypothetical protein	-
LO012_RS20000	32082..32786	-	705	WP_001067856.1	IS6-like element IS26 family transposase	-
LO012_RS20005	32916..33731	+	816	WP_000018326.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-
LO012_RS20010	33885..34064	-	180	WP_000902128.1	hypothetical protein	-
LO012_RS20015	34156..34860	-	705	WP_001067856.1	IS6-like element IS26 family transposase	-
LO012_RS20020	34990..35805	+	816	WP_000018326.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-
LO012_RS20025	35959..36138	-	180	WP_000902128.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58 / aph(3')-Ia	-	1..37306	37306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T225348 WP_000438826.1 NZ_CP087318:c31473-31186 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T225348 NZ_CP121137:c3429103-3428570 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTAGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT225348 WP_001983304.1 NZ_CP087318:c31199-30885 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT225348 NZ_CP121137:c3429380-3429114 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: N9M5I3