Detailed information of TA system TA225330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 56085..56632
• Replicon: plasmid pE956_1
• Accession: NZ_CP087295
• Organism: Escherichia coli strain APEC E956

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: LO740_RS23950
• Protein ID: WP_001384452.1
• Coordinates: 56085..56363 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: LO740_RS23955
• Protein ID: WP_000079941.1
• Coordinates: 56363..56632 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO740_RS23925 (51797)	51797..52117	-	321	Protein_47	IS66 family insertion sequence element accessory protein TnpB	-
LO740_RS23930 (52114)	52114..52518	-	405	WP_000839181.1	transposase	-
LO740_RS23935 (52768)	52768..53103	+	336	WP_001080729.1	colicin 1A immunity protein	-
LO740_RS23940 (53125)	53125..55005	-	1881	WP_001283335.1	colicin Ia central receptor-binding domain-containing protein	-
LO740_RS23945 (55191)	55191..56039	-	849	WP_001057989.1	3'-5' exonuclease	-
LO740_RS23950 (56085)	56085..56363	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
LO740_RS23955 (56363)	56363..56632	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
LO740_RS23960 (57548)	57548..58405	-	858	WP_000130940.1	incFII family plasmid replication initiator RepA	-
LO740_RS23965 (58398)	58398..58445	-	48	WP_077822033.1	hypothetical protein	-
LO740_RS23970 (58436)	58436..58672	+	237	WP_223368648.1	replication protein RepA	-
LO740_RS23975 (58698)	58698..58958	-	261	WP_000083818.1	replication regulatory protein RepA	-
LO740_RS23980 (59048)	59048..59404	-	357	Protein_58	thermonuclease family protein	-
LO740_RS23985 (59647)	59647..59859	-	213	WP_001311693.1	hypothetical protein	-
LO740_RS23990 (59997)	59997..60557	-	561	WP_000139359.1	fertility inhibition protein FinO	-
LO740_RS23995 (60612)	60612..61357	-	746	Protein_61	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..138857	138857

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T225330 WP_001384452.1 NZ_CP087295:c56363-56085 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T225330 NZ_CP121133:c4942714-4942346 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT225330 WP_000079941.1 NZ_CP087295:c56632-56363 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT225330 NZ_CP121133:c4942932-4942711 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A829CL36

Antitoxin

• AlphaFold DB: A0A629TXC5