Detailed information of TA system TA225322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4367177..4367808
• Replicon: chromosome
• Accession: NZ_CP087294
• Organism: Escherichia coli strain APEC E956

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: LO740_RS21270
• Protein ID: WP_001260301.1
• Coordinates: 4367533..4367808 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: LO740_RS21265
• Protein ID: WP_000593557.1
• Coordinates: 4367177..4367536 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO740_RS21240 (4363309)	4363309..4364253	+	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-
LO740_RS21245 (4364250)	4364250..4365083	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
LO740_RS21250 (4365083)	4365083..4365847	+	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
LO740_RS21255 (4365844)	4365844..4366650	+	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
LO740_RS21260 (4366656)	4366656..4367057	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
LO740_RS21265 (4367177)	4367177..4367536	-	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LO740_RS21270 (4367533)	4367533..4367808	-	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
LO740_RS21275 (4367881)	4367881..4369005	-	1125	WP_001318088.1	ABC transporter permease	-
LO740_RS21280 (4369005)	4369005..4371740	-	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
LO740_RS21285 (4371737)	4371737..4372804	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T225322 WP_001260301.1 NZ_CP087294:c4367808-4367533 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T225322 NZ_CP121133:351429-352346 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT225322 WP_000593557.1 NZ_CP087294:c4367536-4367177 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT225322 NZ_CP121133:350665-351423 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAGCCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1