Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3238953..3239578 Replicon chromosome
Accession NZ_CP087294
Organism Escherichia coli strain APEC E956

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag LO740_RS15825 Protein ID WP_000911329.1
Coordinates 3238953..3239351 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag LO740_RS15830 Protein ID WP_000450524.1
Coordinates 3239351..3239578 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO740_RS15805 (3234860) 3234860..3235060 + 201 WP_000383836.1 YpfN family protein -
LO740_RS15810 (3235141) 3235141..3235839 - 699 WP_000679819.1 esterase -
LO740_RS15815 (3235913) 3235913..3237928 - 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
LO740_RS15820 (3237943) 3237943..3238806 - 864 WP_001267498.1 neutral zinc metallopeptidase -
LO740_RS15825 (3238953) 3238953..3239351 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
LO740_RS15830 (3239351) 3239351..3239578 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
LO740_RS15835 (3239735) 3239735..3240448 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
LO740_RS15840 (3240661) 3240661..3241695 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
LO740_RS15845 (3241712) 3241712..3242590 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
LO740_RS15850 (3242736) 3242736..3243308 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
LO740_RS15855 (3243308) 3243308..3243778 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T225317 WP_000911329.1 NZ_CP087294:c3239351-3238953 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T225317 NZ_CP121131:3871056-3871424 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT225317 WP_000450524.1 NZ_CP087294:c3239578-3239351 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT225317 NZ_CP121131:3870838-3871059 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References