Detailed information of TA system TA225307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2167096..2168678
• Replicon: chromosome
• Accession: NZ_CP087294
• Organism: Escherichia coli strain APEC E956

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0J2BJD7
• Locus tag: LO740_RS10500
• Protein ID: WP_001125469.1
• Coordinates: 2167096..2168412 (-)
• Length: 439 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LO740_RS10505
• Protein ID: WP_001296726.1
• Coordinates: 2168412..2168678 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO740_RS10485 (2164485)	2164485..2165195	-	711	WP_001195165.1	fimbria/pilus periplasmic chaperone	-
LO740_RS10490 (2165557)	2165557..2166120	-	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
LO740_RS10495 (2166554)	2166554..2166820	-	267	Protein_2046	AraC family transcriptional regulator	-
LO740_RS10500 (2167096)	2167096..2168412	-	1317	WP_001125469.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LO740_RS10505 (2168412)	2168412..2168678	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LO740_RS10510 (2168871)	2168871..2170760	-	1890	Protein_2049	autotransporter barrel domain-containing lipoprotein	-
LO740_RS10515 (2170910)	2170910..2172502	-	1593	WP_000113152.1	autoinducer-2 kinase	-
LO740_RS10520 (2172581)	2172581..2173534	-	954	WP_000154356.1	transcriptional regulator LsrR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 439 a.a.
• Molecular weight: 49275.91 Da
• Isoelectric Point: 8.8783

>T225307 WP_001125469.1 NZ_CP087294:c2168412-2167096 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY

Nucleotide

• Length: 1317 bp

>T225307 NZ_CP121129:5239554-5239937 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT225307 WP_001296726.1 NZ_CP087294:c2168678-2168412 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT225307 NZ_CP121129:5239312-5239554 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0J2BJD7