Detailed information of TA system TA225306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2080121..2080757
• Replicon: chromosome
• Accession: NZ_CP087294
• Organism: Escherichia coli strain APEC E956

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: LO740_RS10130
• Protein ID: WP_000813796.1
• Coordinates: 2080121..2080297 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LO740_RS10135
• Protein ID: WP_076838470.1
• Coordinates: 2080341..2080757 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO740_RS10110 (2075743)	2075743..2076915	-	1173	WP_001236216.1	BenE family transporter YdcO	-
LO740_RS10115 (2077007)	2077007..2077543	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
LO740_RS10120 (2077616)	2077616..2079577	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LO740_RS10125 (2079669)	2079669..2079899	-	231	WP_000494243.1	YncJ family protein	-
LO740_RS10130 (2080121)	2080121..2080297	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LO740_RS10135 (2080341)	2080341..2080757	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LO740_RS10140 (2080836)	2080836..2082242	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
LO740_RS10145 (2082487)	2082487..2083632	+	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
LO740_RS10150 (2083650)	2083650..2084663	+	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
LO740_RS10155 (2084664)	2084664..2085605	+	942	WP_001251307.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T225306 WP_000813796.1 NZ_CP087294:2080121-2080297 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T225306 NZ_CP121129:c4961908-4961423 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATGCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT225306 WP_076838470.1 NZ_CP087294:2080341-2080757 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT225306 NZ_CP121129:c4962162-4961896 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3