Detailed information of TA system TA225305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1773125..1773909
• Replicon: chromosome
• Accession: NZ_CP087294
• Organism: Escherichia coli strain APEC E956

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LO740_RS08580
• Protein ID: WP_000613624.1
• Coordinates: 1773125..1773619 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: LO740_RS08585
• Protein ID: WP_001110446.1
• Coordinates: 1773616..1773909 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO740_RS08560 (1768318)	1768318..1769415	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
LO740_RS08565 (1769415)	1769415..1770356	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
LO740_RS08570 (1770422)	1770422..1772065	+	1644	WP_000096482.1	flagellar hook-associated protein FlgK	-
LO740_RS08575 (1772077)	1772077..1773030	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
LO740_RS08580 (1773125)	1773125..1773619	-	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
LO740_RS08585 (1773616)	1773616..1773909	-	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
LO740_RS08590 (1774043)	1774043..1777228	-	3186	WP_000827395.1	ribonuclease E	-
LO740_RS08595 (1777801)	1777801..1778760	+	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T225305 WP_000613624.1 NZ_CP087294:c1773619-1773125 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T225305 NZ_CP121129:c4835062-4834694 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT225305 WP_001110446.1 NZ_CP087294:c1773909-1773616 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT225305 NZ_CP121129:c4835280-4835059 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829G775