Detailed information of TA system TA225304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1525843..1526548
• Replicon: chromosome
• Accession: NZ_CP087294
• Organism: Escherichia coli strain APEC E956

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LO740_RS07390
• Protein ID: WP_000539521.1
• Coordinates: 1526162..1526548 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LO740_RS07385
• Protein ID: WP_001280945.1
• Coordinates: 1525843..1526172 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO740_RS07370 (1521000)	1521000..1521911	+	912	WP_274491401.1	glutathione ABC transporter permease GsiD	-
LO740_RS07375 (1522089)	1522089..1524437	+	2349	Protein_1430	EAL domain-containing protein	-
LO740_RS07380 (1524445)	1524445..1525773	+	1329	WP_000086919.1	GGDEF domain-containing protein	-
LO740_RS07385 (1525843)	1525843..1526172	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LO740_RS07390 (1526162)	1526162..1526548	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO740_RS07395 (1526774)	1526774..1528099	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LO740_RS07400 (1528312)	1528312..1528695	+	384	WP_000497137.1	biofilm formation regulator BssR	-
LO740_RS07405 (1528806)	1528806..1529921	+	1116	WP_274491402.1	aldose sugar dehydrogenase YliI	-
LO740_RS07410 (1529918)	1529918..1530544	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T225304 WP_000539521.1 NZ_CP087294:c1526548-1526162 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T225304 NZ_CP121129:c4809096-4808749 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAATCGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT225304 WP_001280945.1 NZ_CP087294:c1526172-1525843 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT225304 NZ_CP121129:c4808749-4808450 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6