Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 284401..284898 Replicon chromosome
Accession NZ_CP087294
Organism Escherichia coli strain APEC E956

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LO740_RS01315 Protein ID WP_001173343.1
Coordinates 284725..284898 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag LO740_RS01310 Protein ID WP_001317561.1
Coordinates 284401..284697 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO740_RS01290 (281133) 281133..281852 - 720 WP_000611288.1 two-component system response regulator DcuR -
LO740_RS01295 (281849) 281849..283480 - 1632 WP_001216477.1 sensor histidine kinase -
LO740_RS01300 (283660) 283660..283890 + 231 WP_000371717.1 4Fe-4S mono-cluster protein YjdI -
LO740_RS01305 (283902) 283902..284174 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
LO740_RS01310 (284401) 284401..284697 + 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LO740_RS01315 (284725) 284725..284898 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LO740_RS01320 (285017) 285017..286534 - 1518 WP_000003805.1 lysine--tRNA ligase -
LO740_RS01325 (286771) 286771..288228 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T225299 WP_001173343.1 NZ_CP087294:284725-284898 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T225299 NZ_CP121129:519202-519735 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTAGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT225299 WP_001317561.1 NZ_CP087294:284401-284697 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT225299 NZ_CP121129:518925-519191 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References