Detailed information of TA system TA225290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 88396..88931
• Replicon: plasmid p102026_1
• Accession: NZ_CP087291
• Organism: Escherichia coli strain APEC 102026

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: LO741_RS26070
• Protein ID: WP_000222766.1
• Coordinates: 88396..88683 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: LO741_RS26075
• Protein ID: WP_001132900.1
• Coordinates: 88680..88931 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO741_RS26065 (86914)	86914..88127	+	1214	WP_191630560.1	IS3 family transposase	-
LO741_RS26070 (88396)	88396..88683	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO741_RS26075 (88680)	88680..88931	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LO741_RS26080 (89894)	89894..90751	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
LO741_RS26085 (90744)	90744..91226	-	483	WP_001273588.1	hypothetical protein	-
LO741_RS26090 (91219)	91219..91266	-	48	WP_229471593.1	hypothetical protein	-
LO741_RS26095 (91257)	91257..91508	+	252	WP_223195197.1	replication protein RepA	-
LO741_RS26100 (91525)	91525..91782	-	258	WP_000083845.1	replication regulatory protein RepA	-
LO741_RS26105 (92066)	92066..92215	-	150	WP_001312851.1	Hok/Gef family protein	-
- (92259)	92259..92320	+	62	NuclAT_1	-	-
- (92259)	92259..92320	+	62	NuclAT_1	-	-
- (92259)	92259..92320	+	62	NuclAT_1	-	-
- (92259)	92259..92320	+	62	NuclAT_1	-	-
LO741_RS26110 (92576)	92576..92650	-	75	Protein_89	endonuclease	-
LO741_RS26115 (92896)	92896..93108	-	213	WP_001299730.1	ANR family transcriptional regulator	-
LO741_RS26120 (93245)	93245..93805	-	561	WP_000139315.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..174489	174489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T225290 WP_000222766.1 NZ_CP087291:c88683-88396 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T225290 NZ_CP121127:c4385723-4385439 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTTAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT225290 WP_001132900.1 NZ_CP087291:c88931-88680 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT225290 NZ_CP121127:c4385955-4385713 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCTGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2

Antitoxin

• AlphaFold DB: V0VIP2