Detailed information of TA system TA225288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 51023..51666
• Replicon: plasmid p102026_1
• Accession: NZ_CP087291
• Organism: Escherichia coli strain APEC 102026

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: LO741_RS25905
• Protein ID: WP_001044768.1
• Coordinates: 51250..51666 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: LO741_RS25900
• Protein ID: WP_001261287.1
• Coordinates: 51023..51253 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO741_RS25865 (46239)	46239..46406	+	168	Protein_40	colicin-B	-
LO741_RS25870 (46424)	46424..46950	-	527	Protein_41	colicin B immunity protein	-
LO741_RS25875 (47194)	47194..48009	+	816	WP_001312845.1	lipid II-degrading bacteriocin colicin M	-
LO741_RS25880 (48059)	48059..48412	-	354	WP_000864812.1	colicin M immunity protein	-
LO741_RS25885 (48590)	48590..49381	-	792	WP_000016494.1	site-specific integrase	-
LO741_RS25890 (49378)	49378..50067	-	690	WP_000796229.1	hypothetical protein	-
LO741_RS25895 (50111)	50111..50461	-	351	WP_000493379.1	hypothetical protein	-
LO741_RS25900 (51023)	51023..51253	+	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LO741_RS25905 (51250)	51250..51666	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
LO741_RS25910 (51828)	51828..53966	-	2139	WP_000350635.1	AAA family ATPase	-
LO741_RS25915 (54324)	54324..55495	+	1172	WP_094284492.1	IS91 family transposase	-
LO741_RS25920 (55658)	55658..55897	-	240	WP_001105068.1	hypothetical protein	-
LO741_RS25925 (56003)	56003..56278	-	276	WP_000421266.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LO741_RS25930 (56278)	56278..56562	-	285	WP_012006537.1	ribbon-helix-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..174489	174489
	-	inside	IScluster/Tn	-	-	54668..73764	19096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T225288 WP_001044768.1 NZ_CP087291:51250-51666 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T225288 NZ_CP121127:3659488-3659706 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT225288 WP_001261287.1 NZ_CP087291:51023-51253 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT225288 NZ_CP121127:3659087-3659461 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4