Detailed information of TA system TA225278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4749970..4750606
• Replicon: chromosome
• Accession: NZ_CP087290
• Organism: Escherichia coli strain APEC 102026

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: LO741_RS23345
• Protein ID: WP_000813796.1
• Coordinates: 4749970..4750146 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LO741_RS23350
• Protein ID: WP_076838470.1
• Coordinates: 4750190..4750606 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO741_RS23325 (4745592)	4745592..4746764	-	1173	WP_001236216.1	BenE family transporter YdcO	-
LO741_RS23330 (4746856)	4746856..4747392	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
LO741_RS23335 (4747465)	4747465..4749426	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LO741_RS23340 (4749518)	4749518..4749748	-	231	WP_000494243.1	YncJ family protein	-
LO741_RS23345 (4749970)	4749970..4750146	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LO741_RS23350 (4750190)	4750190..4750606	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LO741_RS23355 (4750685)	4750685..4752091	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
LO741_RS23360 (4752336)	4752336..4753481	+	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
LO741_RS23365 (4753499)	4753499..4754512	+	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
LO741_RS23370 (4754513)	4754513..4755454	+	942	WP_001251307.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T225278 WP_000813796.1 NZ_CP087290:4749970-4750146 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T225278 NZ_CP121109:c534995-534612 [Pantoea sp. X85]
ATGGCAATTTATGTCTTAAAGCCATTCGATAGAAACACCAAAGGTGACGCTATCAATAGTGCAAAATTATGCAAAGCGGC
GCTGGAAGTCATGGCAGGGATTTATGAAGCCAGTTTCGGCAGGGGAGTTTACAAGAAGCGCATTCCTTTAGTTGCTGGAA
AAAGTGGCGGGGCCCGGGCGGTTGTAGCCTTTAAAACTGAAAAACATCTTTTTTTCGTCAACGGCTATGCTAAATCCGCA
TTCAAAAGTAGTGGTCGAGAGATATCTGAATCTGATCTCGCTCTCTACAAGGAAGTGGCCAAACAACTGTTTGAAATGAC
ATCAGAACAAGCAAAAGTAGCCATCAATACTGGCAAGATGCGTGAGGTAAAATGTGATGGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT225278 WP_076838470.1 NZ_CP087290:4750190-4750606 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT225278 NZ_CP121109:c534619-534293 [Pantoea sp. X85]
ATGGTTAATCATCTGAAAGATTTACAAGAGATCGCCAGGGATTTTAATGAAATTGGCGTGGTCACCGATGAGCTAAAAGA
AAAAATTGATTCTCGTGTGAGATTGCGTGAATTGCGCGCTTCGCTACCTGTCCTGCACATGATGTCGGGAGATGAAATAA
ATCAGTTACGTCAACGTTATAATCTGAGCCAGGCGATGCTGGCCGCCTACGTCGGCATGTCAGTGGGGACGGTAGTGAAG
TGGGAACGAGAAGAGAAAAAACCTAACGGAGCAGCACTGCGATTGCTCAATCTTATCGAGCGAAAGGGGTTGGAAGTGCT
GCAATAA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3