Detailed information of TA system TA225273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4388488..4389272
• Replicon: chromosome
• Accession: NZ_CP087290
• Organism: Escherichia coli strain APEC 102026

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LO741_RS21475
• Protein ID: WP_000613624.1
• Coordinates: 4388488..4388982 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: LO741_RS21480
• Protein ID: WP_001110446.1
• Coordinates: 4388979..4389272 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO741_RS21455 (4383681)	4383681..4384778	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
LO741_RS21460 (4384778)	4384778..4385719	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
LO741_RS21465 (4385785)	4385785..4387428	+	1644	WP_000096482.1	flagellar hook-associated protein FlgK	-
LO741_RS21470 (4387440)	4387440..4388393	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
LO741_RS21475 (4388488)	4388488..4388982	-	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
LO741_RS21480 (4388979)	4388979..4389272	-	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
LO741_RS21485 (4389406)	4389406..4392591	-	3186	WP_000827395.1	ribonuclease E	-
LO741_RS21490 (4393164)	4393164..4394123	+	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T225273 WP_000613624.1 NZ_CP087290:c4388982-4388488 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T225273 NZ_CP121108:c1108704-1108486 [Pantoea sp. X85]
ATGAGCAACCAAGCCTTAACTAAAACAGATTATTTGATGCGACTGCGCCGCTGTCGCTCAATCGATACCCTTGAACGGGT
TATCGAAAAAAACAAGTATGAATTACCTGATAATGAACTCGCGGTATTCTATTCGGCAGCCGATCATCGTCTGGCTGAAC
TGACAATGAATAAGCTTTACGATAAAGTACCGGTTTCTGTCTGGAAGTTCGTCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT225273 WP_001110446.1 NZ_CP087290:c4389272-4388979 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT225273 NZ_CP121108:c1109106-1108729 [Pantoea sp. X85]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTGTGTGAGAACCTGTTCGACGAAAGTATGGC
GACATTGACCGACAGCCATCATGGTTGGGTTAATGATCCTACTTCGGAAAGCAATTTACAGCTCAACGATTTGATTGAGC
ATATCGCTTCTTTCACGATGAATTACAAAATTAAGCACGTTGAAGATGAAGCCCTGATTACGCAAATCGATGAATATCTT
GACGATACCTTTATGCTGTTCAGCAGCTATGGAATCAATACTCAGGATCTACAACGGTGGCAGCGTTCAGCAAAACGCTT
ATTTAATCTGTTTGCCGAAGAGTGCGCTTATCTCCAACAACCTAGCCATTCCTTTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829G775