Detailed information of TA system TA225272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4137349..4138054
• Replicon: chromosome
• Accession: NZ_CP087290
• Organism: Escherichia coli strain APEC 102026

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LO741_RS20260
• Protein ID: WP_000539521.1
• Coordinates: 4137668..4138054 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LO741_RS20255
• Protein ID: WP_001280945.1
• Coordinates: 4137349..4137678 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO741_RS20240 (4132506)	4132506..4133417	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
LO741_RS20245 (4133595)	4133595..4135943	+	2349	Protein_3943	EAL domain-containing protein	-
LO741_RS20250 (4135951)	4135951..4137279	+	1329	WP_000086919.1	GGDEF domain-containing protein	-
LO741_RS20255 (4137349)	4137349..4137678	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LO741_RS20260 (4137668)	4137668..4138054	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO741_RS20265 (4138280)	4138280..4139605	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LO741_RS20270 (4139818)	4139818..4140201	+	384	WP_000497137.1	biofilm formation regulator BssR	-
LO741_RS20275 (4140312)	4140312..4141427	+	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
LO741_RS20280 (4141424)	4141424..4142050	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T225272 WP_000539521.1 NZ_CP087290:c4138054-4137668 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T225272 NZ_CP121108:c937018-936707 [Pantoea sp. X85]
ATGCACGTTATCTCAAGGAAAGTGTTTACAGAAGCGACACGGAGTTTTCCAAATGATGCAGCAGCATTGGATGCCACTTA
TCGAACTTTAAATGGCAACGATTTTGCCGATCCAGATGCGCTCAGAGCCTTTTTTCCAAGTCTTGACCGAATGAAATACC
GGGCGAAATGGTGGGTGATTAACGTAGGCGGAAATAATCTCAGAGTGATGTTTTTTGCTGATTTTATAGCCCAACGAATC
TTCATTAAACACGTTGTAACGCATGCCGAGTATGACAAATGGGTAAAGCATTATCGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT225272 WP_001280945.1 NZ_CP087290:c4137678-4137349 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT225272 NZ_CP121108:c936710-936294 [Pantoea sp. X85]
ATGATTGCCGAAGCCATCCAGGCCAGCAACAATCTGATTAAAGCCGTCCCGCTGCTGGGCGGAAGTAACGCCAAAGCTGA
TTATGAGCAGGCGTTAGAACTGGTGGAGTATCTGATTGAACATCAACCTGATCATCCGCTGATTGAAATGCTCACCGATA
AAGTGGCGAAATATGAAGATAGTGCGCCGGAATTTGCTGCGTTCAACGCGCGTGTCACTGCATTACCTGGCGGTGTCGCG
TTACTGCGGGTGTTGATGGATCAACATAATCTCAATCAGTCATCCTTCGTGAATGAAATTGGTCAGCGTTCTTATGTAAA
CCGAATTTTGAAAGGAGAACGTTCGTTGACAGACAAACACAAAGCCGCGCTGGCTAAACGTTTTAACCTGCCTTTCGAAG
CGTTTCGCGAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6