Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3714438..3715056 Replicon chromosome
Accession NZ_CP087290
Organism Escherichia coli strain APEC 102026

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LO741_RS18025 Protein ID WP_001291435.1
Coordinates 3714438..3714656 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LO741_RS18030 Protein ID WP_000344800.1
Coordinates 3714682..3715056 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO741_RS17990 (3709727) 3709727..3710299 + 573 WP_000779821.1 YbaY family lipoprotein -
LO741_RS17995 (3710330) 3710330..3710641 - 312 WP_000409911.1 MGMT family protein -
LO741_RS18005 (3711020) 3711020..3711373 + 354 WP_000878140.1 DUF1428 domain-containing protein -
LO741_RS18010 (3711415) 3711415..3712965 - 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LO741_RS18015 (3713129) 3713129..3713599 - 471 WP_000136192.1 YlaC family protein -
LO741_RS18020 (3713715) 3713715..3714266 - 552 WP_001471141.1 maltose O-acetyltransferase -
LO741_RS18025 (3714438) 3714438..3714656 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LO741_RS18030 (3714682) 3714682..3715056 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LO741_RS18035 (3715602) 3715602..3718751 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LO741_RS18040 (3718774) 3718774..3719967 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225270 WP_001291435.1 NZ_CP087290:c3714656-3714438 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T225270 NZ_CP121108:134901-135194 [Pantoea sp. X85]
ATGCAAACGGAACTGGAATTTGAGTGGGACCTTAGCAAAGCAGAGTCTAATCACCGCAAGCATGGCATCCGCTTCGAAAT
TGCCGTGCGCGTGTTTGATGATCCTTTTCATCTGTCTAGCCAGGATCGTTGTGAGAATGGCGAATATCGCTGGCAATCAA
TAGGTAATGTTATGGGCCATATTATTATTTTGGTGGCCCACACGGTACGTTTCGAGCAGGGCACTGAGATTATTCGCATC
ATTAGTGCACGTCCTGCCACGCGCAATGAGAGGAGACGCTATGAGCAAGGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT225270 WP_000344800.1 NZ_CP087290:c3715056-3714682 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT225270 NZ_CP121108:135181-135483 [Pantoea sp. X85]
ATGAGCAAGGTTAGGTTTAAACGCAGCGAATTACCGCCGCTTTCGGATAGCCTGCAATCTGAACTGGACGCGCTGAGTCA
AAAGCCGGATAGCGAGATTGATTGCGGTGAAATCGCCCCGTTAAATGAACGTTTCTGGAACAGTGCTTCCAGCGGGCGAT
TTTATCGTCCGATTAAAACACAGGCTTCAGTCAGAATTGACGCGGATGTGCTTGAGTGGCTGAAAGCGCCGGGTAAAGGG
TACCAGACGCGTTTGAACGCCATTTTGCGCGCGGCGATGCTGCGCGAATTAGAAAGCAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References