Detailed information of TA system TA225258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1981278..1981909
• Replicon: chromosome
• Accession: NZ_CP087290
• Organism: Escherichia coli strain APEC 102026

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: LO741_RS09650
• Protein ID: WP_001260301.1
• Coordinates: 1981634..1981909 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: LO741_RS09645
• Protein ID: WP_000593557.1
• Coordinates: 1981278..1981637 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO741_RS09620 (1977408)	1977408..1978352	+	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-
LO741_RS09625 (1978349)	1978349..1979182	+	834	WP_021539320.1	nickel ABC transporter permease subunit NikC	-
LO741_RS09630 (1979182)	1979182..1979946	+	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
LO741_RS09635 (1979943)	1979943..1980749	+	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
LO741_RS09640 (1980755)	1980755..1981156	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
LO741_RS09645 (1981278)	1981278..1981637	-	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LO741_RS09650 (1981634)	1981634..1981909	-	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
LO741_RS09655 (1981982)	1981982..1983106	-	1125	WP_001318088.1	ABC transporter permease	-
LO741_RS09660 (1983106)	1983106..1985841	-	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
LO741_RS09665 (1985838)	1985838..1986905	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T225258 WP_001260301.1 NZ_CP087290:c1981909-1981634 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T225258 NZ_CP121087:c2679408-2679235 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT225258 WP_000593557.1 NZ_CP087290:c1981637-1981278 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT225258 NZ_CP121087:c2679732-2679436 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1