Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1172532..1173186 Replicon chromosome
Accession NZ_CP087290
Organism Escherichia coli strain APEC 102026

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LO741_RS05445 Protein ID WP_000244781.1
Coordinates 1172532..1172939 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LO741_RS05450 Protein ID WP_000354046.1
Coordinates 1172920..1173186 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO741_RS05425 (1168489) 1168489..1170222 - 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -
LO741_RS05430 (1170228) 1170228..1170938 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LO741_RS05435 (1170963) 1170963..1171859 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LO741_RS05440 (1171971) 1171971..1172492 + 522 WP_001055874.1 flavodoxin FldB -
LO741_RS05445 (1172532) 1172532..1172939 - 408 WP_000244781.1 protein YgfX Toxin
LO741_RS05450 (1172920) 1172920..1173186 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LO741_RS05455 (1173439) 1173439..1174419 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
LO741_RS05460 (1174496) 1174496..1175155 - 660 WP_000250269.1 hemolysin III family protein -
LO741_RS05465 (1175319) 1175319..1175630 - 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
LO741_RS05470 (1175675) 1175675..1177108 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T225253 WP_000244781.1 NZ_CP087290:c1172939-1172532 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T225253 NZ_CP121087:2332728-2332880 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT225253 WP_000354046.1 NZ_CP087290:c1173186-1172920 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT225253 NZ_CP121087:c2332679-2332622 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References