Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 168507..169150 Replicon plasmid p16_1068_1
Accession NZ_CP087288
Organism Escherichia coli strain APEC 16-1068

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag LO742_RS26020 Protein ID WP_001034046.1
Coordinates 168734..169150 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag LO742_RS26015 Protein ID WP_001261278.1
Coordinates 168507..168737 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO742_RS25985 (164019) 164019..164324 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
LO742_RS25990 (164326) 164326..164544 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
LO742_RS25995 (165134) 165134..165622 + 489 WP_011254646.1 hypothetical protein -
LO742_RS26000 (165656) 165656..166789 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
LO742_RS26005 (166956) 166956..167729 - 774 WP_000905949.1 hypothetical protein -
LO742_RS26010 (167742) 167742..168242 - 501 WP_000528932.1 HEPN family nuclease -
LO742_RS26015 (168507) 168507..168737 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LO742_RS26020 (168734) 168734..169150 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
LO742_RS26025 (169195) 169195..171609 - 2415 WP_274431392.1 hypothetical protein -
LO742_RS26030 (171686) 171686..172987 - 1302 WP_246863463.1 hypothetical protein -
LO742_RS26035 (173368) 173368..173598 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
LO742_RS26040 (173595) 173595..174011 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..195721 195721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T225248 WP_001034046.1 NZ_CP087288:168734-169150 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T225248 NZ_CP121087:904951-905058 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT225248 WP_001261278.1 NZ_CP087288:168507-168737 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT225248 NZ_CP121087:c904898-904837 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References