Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 164019..164544 Replicon plasmid p16_1068_1
Accession NZ_CP087288
Organism Escherichia coli strain APEC 16-1068

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag LO742_RS25985 Protein ID WP_001159871.1
Coordinates 164019..164324 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag LO742_RS25990 Protein ID WP_000813630.1
Coordinates 164326..164544 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO742_RS25970 (159975) 159975..161180 - 1206 WP_001442122.1 AAA family ATPase -
LO742_RS25975 (161801) 161801..162532 + 732 WP_000504262.1 replication initiation protein -
LO742_RS25980 (163212) 163212..164018 - 807 WP_021543129.1 site-specific integrase -
LO742_RS25985 (164019) 164019..164324 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
LO742_RS25990 (164326) 164326..164544 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LO742_RS25995 (165134) 165134..165622 + 489 WP_011254646.1 hypothetical protein -
LO742_RS26000 (165656) 165656..166789 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
LO742_RS26005 (166956) 166956..167729 - 774 WP_000905949.1 hypothetical protein -
LO742_RS26010 (167742) 167742..168242 - 501 WP_000528932.1 HEPN family nuclease -
LO742_RS26015 (168507) 168507..168737 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
LO742_RS26020 (168734) 168734..169150 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..195721 195721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T225247 WP_001159871.1 NZ_CP087288:c164324-164019 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T225247 NZ_CP121087:c728650-728474 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT225247 WP_000813630.1 NZ_CP087288:c164544-164326 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT225247 NZ_CP121087:c728428-728012 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References