Detailed information of TA system TA225243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 119679..120304
• Replicon: plasmid p16_1068_1
• Accession: NZ_CP087288
• Organism: Escherichia coli strain APEC 16-1068

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LO742_RS25720
• Protein ID: WP_000911333.1
• Coordinates: 119906..120304 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: LO742_RS25715
• Protein ID: WP_000450520.1
• Coordinates: 119679..119906 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO742_RS25715 (119679)	119679..119906	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LO742_RS25720 (119906)	119906..120304	+	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LO742_RS25725 (120313)	120313..122466	-	2154	WP_000009379.1	type IV conjugative transfer system coupling protein TraD	-
LO742_RS25730 (122719)	122719..123450	-	732	WP_000850416.1	conjugal transfer complement resistance protein TraT	-
LO742_RS25735 (123464)	123464..123973	-	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..195721	195721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T225243 WP_000911333.1 NZ_CP087288:119906-120304 [Escherichia coli]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T225243 NZ_CP121077:50117-50422 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT225243 WP_000450520.1 NZ_CP087288:119679-119906 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT225243 NZ_CP121077:49897-50115 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2